OBCAM CRISPR/Cas9 KO Plasmid (h): sc-405750
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OBCAM CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OBCAM genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OBCAM CRISPR/Cas9 KO Plasmid (h) | sc-405750 | 20 µg | $397.00 |
Overview
OPCML encodes OBCAM, a glycosylphosphatidylinositol (GPI)-anchored immunoglobulin superfamily cell adhesion molecule enriched at the cell surface where it supports cell–cell recognition and tissue architecture. OBCAM participates in contact-dependent signaling by organizing membrane microdomains and modulating receptor tyrosine kinase and integrin-associated pathways that influence cytoskeletal dynamics, migration, and survival programs. In human biology, altered OPCML/OBCAM expression and epigenetic regulation have been linked to disrupted adhesion and aberrant growth signaling across multiple tumor contexts and to changes in neuronal connectivity. These properties make OPCML a useful node for studying adhesion-dependent signal transduction, membrane compartmentalization, and microenvironment-driven phenotypes.
OBCAM CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OPCML gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OPCML together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OPCML open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OBCAM protein expression.
This CRISPR knockout system enables efficient generation of OPCML-deficient cell models for investigation of OBCAM signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OPCML exon(s) critical for OBCAM function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OPCML genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OBCAM CRISPR/Cas9 KO Plasmid (h) and OBCAM CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OPCML locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OBCAM HDR Plasmid (h) and OBCAM HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OPCML homology arms to support homology-directed repair at defined OPCML target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.