MMP2 Antibody (8B4): sc-13595

MMP-2 Antibody (8B4) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect activated human MMP-2, as well as MMP-2 from rat and mouse origins, across applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MMP-2 monoclonal antibody (8B4) is raised against activated MMP-2, ensuring precise binding, and comes in non-conjugated and conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates for diverse experimental needs. Matrix metalloproteinase-2 (MMP-2), also known as gelatinase A, CLG4, MONA, CLG4A, and TBE-1, is a key enzyme within the matrix metalloproteinase family that primarily resides in the extracellular matrix (ECM), where MMP-2 plays a crucial role in degrading ECM components including collagen types IV, V, VII, and X, as well as gelatin type I. MMP-2′s extracellular localization enables tissue remodeling, angiogenesis, and wound healing by facilitating ECM barrier breakdown and cellular migration. This localization becomes critical in cancer metastasis, where MMP-2-mediated ECM degradation enables cancer cell invasion and spread. MMP-2 also modulates bioactive ECM molecules, influencing cell signaling pathways governing proliferation, differentiation, and apoptosis. Ras signaling pathway regulates MMP-2 activation and secretion, which require calcium and zinc ions for catalytic activity. Understanding MMP-2′s localization and regulation remains vital for studying mechanisms in cardiovascular disorders, autoimmune diseases, and inflammatory conditions.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MMP2 Antibody (8B4) has 240 citations in a variety of scientific publications.

Primary antibodies like Anti-MMP2 Antibody (8B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MMP2 Antibody (8B4) Specifications

Alternate Product Name: MMP-2

Product Clone Name: 8B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MMP-2

Epitope Species: human

Product Reactivity/Detection: MMP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 240 citations in various scientific publications

Available Conjugates/Forms for MMP2 Antibody (8B4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MMP2 antibody is MMP2 Antibody (2C1): sc-13594.