Anticorpo Insulin (2D11-H5) raccomandato per il rilevamento di Insulin di origine mouse, rat e human in IP, IF, IHC(P) e ELISA

Anticorpo Insulin (2D11-H5): sc-8033

Anticorpo Insulin (2D11-H5) valutazione 4.9 di 5 di 22.
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Schede tecniche

    • Anti-Insulin Antibody 2D11-H5 è un monoclonale di topo IgG1 (kappa light chain) Insulin antibody fornito in 200 µg/ml
    • generato contro aminoacidi 1-84 rappresentanti la lunghezza intera del Insulin di origine porcine
    • Insulin Antibody (2D11-H5) é raccomandato per il rilevamento di Insulin di origine mouse, rat e human in IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse e bovine and porcine
    • Insulin Antibody (2D11-H5) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di Insulin (2D11-H5): sc-8033.

Does this antibody capture proinsulin as well?

Richiesta da: Zoey111
Thank you for your question. Unfortunately, we do not have that information available for this insulin antibody.
Risposta da: Technical Support
Data di pubblicazione: 2020-07-09

How to dilute this antibody? What volumes can i use to arrive at 1:200?

Richiesta da: anjan1983
Thank you for your question. This antibody is provided as 200µg in 1ml (1000µl), which means you have 1µg of antibody per 5µl of solution. So to make a 1:200 dilution, you need to take 1µg (5µl of solution) into 1ml of your diluent (milk/blocking reagent).
Risposta da: Tech Service
Data di pubblicazione: 2019-08-30

what is the volume of primary antibody in the vial? sc-8033 200ug/ml?

Richiesta da: medha2001
Insulin Antibody (2D11-H5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg in a total volume of 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Risposta da: Tech Support Europe
Data di pubblicazione: 2019-08-30

Can this antibody be use to IF(frozen section)?

Richiesta da: Tienfen
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone).
Risposta da: Technical Support
Data di pubblicazione: 2019-04-22

Want to use antibody to distinguish proinsulin and mature insulin,what products are recommended?

Richiesta da: tinglan
Thank you for your question. Please contact our Technical Service Department and we can help recommend antibodies for the detection of proinsulin and mature insulin depending on what species and application(s) you need to use the antibodies in.
Risposta da: Technical Support
Data di pubblicazione: 2019-03-08

antibody: insulin antibody: prmary host : rabbit reactivity : mouse application : western blot conjugation: unconjugated Can i use this antibody for insulin secretion assay by western.

Richiesta da: firdos
Thank you for your question. Insulin Antibody (2D11-H5): sc-8033 is a mouse monoclonal antibody (raised in a mouse host). And it is not currently recommended for use in Western blot.
Risposta da: Technical Support
Data di pubblicazione: 2018-12-21

Can this antibody be used to detect canine insulin?

Richiesta da: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Technical Support 10
Data di pubblicazione: 2017-01-11

What is the recommended protocol for using this antibody in IHC(P)?

Richiesta da: AbPolly
Thank you for your question. For detailed protocol information, please contact our Technical Services Department or refer to our online protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support 10
Data di pubblicazione: 2017-01-11
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Rated 5 di 5 di da GREAT FEATURES. I purchased this antibody two weeks ago. It worked very well on pancreatic section. I will buy this antibody if I need it later. Shirley
Data di pubblicazione: 2019-06-27
Rated 5 di 5 di da Good Antibody for IHC Good signal on European seabass (Dicentrarchus labrax) fixed in Bouin's solution. Diluition 1:50
Data di pubblicazione: 2019-01-16
Rated 5 di 5 di da Immunohistochemistry: excellent results It works fine for Langerhans islets mouse and rat.
Data di pubblicazione: 2018-05-31
Rated 5 di 5 di da Works great! Works in hES cells at 1:50 dilution for immunofluorescence
Data di pubblicazione: 2018-03-01
Rated 5 di 5 di da Good anitibody for ELISA We got 10 ug sample and used for ELISA. It gave a good result, with 1:300 dilution, and can detect ng/ml level human insulin.
Data di pubblicazione: 2018-02-20
Rated 5 di 5 di da Easy to use on FFPE Mouse We received this as a sample. Before purchase a customer asked us to get a sample and work the antibody up. This is a straight forward, very easy, great looking antibody. Use a general protocol, sodium citrate pH6.0 dilution 1:100 - 1:250.
Data di pubblicazione: 2018-02-10
Rated 4 di 5 di da Very good got a free sample and used to perform western blot analysis on mouse hippocampi. The detected band is very clear, and seems specific.
Data di pubblicazione: 2018-02-08
Rated 5 di 5 di da Works beautifully Works beautifully to detect Insulin in murine tissues as confirmed Insulin KO mouse,1:600 dilution been used ,perfect result.
Data di pubblicazione: 2017-07-23
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