Anticorpo HSP 70 (C92F3A-5) raccomandato per il rilevamento di HSP 70 di origine mouse, rat, human, bovine, porcine, canine, Drosophila, C. elegans e e in WB, IP, IF, IHC(P) e FCM

Anticorpo HSP 70 (C92F3A-5): sc-66048

Anticorpo HSP 70 (C92F3A-5) valutazione 4.4 di 5 di 11.
  • y_2022, m_9, d_26, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_it_IT, sid_66048, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 166ms
  • REVIEWS, PRODUCT
loading
5
8
4
1
3
1
2
0
1
1

Schede tecniche

    • HSP 70 Antibody C92F3A-5 è un monoclonale di topo IgG1, citato in 94 pubblicazioni, fornito in 100 µg/ml
    • raised against HSP 70 purified from HeLa cells with epitope mapping to amino acids 436-503
    • raccomandato per il rilevamento di HSP 70 di origine mouse, rat, human, bovine, porcine, canine, Drosophila e C. elegans in WB, IP, IF, IHC(P) e FCM; non cross-reattivo con HSC 70
    • Vedere HSP 70/HSC 70 (W27): sc-24 for HSP 70 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • Contattaci per ricevere un FREE 10 µg sample di HSP 70 (C92F3A-5): sc-66048.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HSP 70 Antibody (C92F3A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with HSP 70 Antibody (C92F3A-5) (see ordering information below).
loading

i bought 4 antibodies(HSP 20, 60, 70, B-actin)i need a detailed western blot protocol for all of them , i.e. the dilutions of the antibodies, the concentration of sample to be loaded on gel, the buffer for extraction of the protein from heart.

Richiesta da: marwa fouad
Thank you for your question. A link to our recommended protocol for Western blotting with our antibodies is provided below. All of the information you requested is included in the protocol. We recommend loading 50 µg protein on the gel. And we recommend using our RIPA Lysis Buffer (sc-24948) for sample preparation. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-02-13

Can I use this antibody for IHC or IF staining on mouse tissue?

Richiesta da: Anna99
This antibody is recommended for detection by IHC and IF. However, to get best results staining mouse tissue with a mouse antibody, it is best to use a direct conjugate. See HSP 70/HSC 70 (W27): sc-24 for HSP 70 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Risposta da: Tech Service 5
Data di pubblicazione: 2017-01-05
  • y_2022, m_9, d_26, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_it_IT, sid_66048, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 119ms
  • QUESTIONS, PRODUCT
Rated 1 di 5 di da 免染 細胞免染を行い、HSP70の局在を確認しました。シグナルが弱く、LAMP1のシグナルは検出できませんでした。
Data di pubblicazione: 2020-12-01
Rated 5 di 5 di da ottimo funzionamento Ho comprato un mese fa questo anticorpo e sono molto soddisfatta
Data di pubblicazione: 2020-05-29
Rated 5 di 5 di da Robust antibody for western blot We have tested a sample of your antibody against HSP70. It worked very well in our first experience. We did an experience with protein extracted from human peripheral blood monocyte cells (B), testis cells (T) and sertoli cells (SC). We used ~150 ug of protein in NuPAGE® Midi 4-12% Bis-Tris gels. Even with an antibody dilution of 1:700 the signal was very strong being saturated upon 1 minute of exposure, with exception for SC. The secondary antibody was an anti-mouse also from Santa Cruz Biotech.
Data di pubblicazione: 2018-03-19
Rated 3 di 5 di da The band is quite weak 1:100 diluted for wb of rat protein ,the band is quite weak!
Data di pubblicazione: 2018-02-13
Rated 5 di 5 di da Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Data di pubblicazione: 2018-01-04
Rated 5 di 5 di da Clear western results Western blot data is great; strong signal with low background
Data di pubblicazione: 2016-12-30
Rated 5 di 5 di da Great bands This antibody is great for Western Blot! Strong bands with clean background.
Data di pubblicazione: 2016-12-23
Rated 5 di 5 di da Works great in WB in human cell lines Works great in WB in human cell lines.
Data di pubblicazione: 2016-01-05
  • y_2022, m_9, d_26, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_it_IT, sid_66048, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 22ms
  • REVIEWS, PRODUCT