Anticorpo HSP 47 (G-12) raccomandato per il rilevamento di HSP 47 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo HSP 47 (G-12): sc-5293

Anticorpo HSP 47 (G-12) valutazione 4.9 di 5 di 13.
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Schede tecniche

    • Anti-HSP 47 Antibody G-12 è un monoclonale di topo IgG2a κ HSP 47 antibody, citato in 14 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 129-300 di HSP 47 di origine human
    • HSP 47 Antibody (G-12) é raccomandato per il rilevamento di HSP 47 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • HSP 47 Antibody (G-12) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di HSP 47 (G-12): sc-5293.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HSP 47 Antibody (G-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with HSP 47 Antibody (G-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Do you have the blocking peptide for HSP 47 Antibody (G-12): sc-5293?

Richiesta da: Manjunath Nayak
Thank you for the question. This antibody was raised against amino acids 129-300 of HSP 47 of human origin. A blocking peptide / recomb. protein fragment with a length of >170 aas would commonly be too long to efficiently block the antibody binding sites of this monoclonal antibody in a competition assay. Unfortunately, we therefore do not offer the corresponding blocking / negative control reagent. Instead, you might want to try a -NIC or CRISPR or siRNA negative control in case you are working with cultured cells.
Risposta da: Technical Support Europe
Data di pubblicazione: 2018-01-31

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Richiesta da: jerojero
Thank you for your question Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Santa Cruz TS
Data di pubblicazione: 2017-01-27
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Rated 5 di 5 di da Perfect antibody against Hsp47 Hsp47 is collagen-specific ER chaperone. We used this antibodies for multiple applications including western blotting, immunocytostaining and immunofluorescence of tissues (paraffin). All staining was specific and strong. Specificity of this antibody was validated using inhibitor of up-stream molecule (site-1 protease). I strongly suggest to purchase this clone from Santa Cruz.
Data di pubblicazione: 2019-08-28
Rated 5 di 5 di da excellent ウエスタンブロットおよび蛍光免疫染色に使用。ノンスペバンドもなく、また低濃度でも十分に検出できます。
Data di pubblicazione: 2019-04-24
Rated 5 di 5 di da Antibodies nicely working for WB Band of appropriate size after immunobloting of LN229 cell lystes.
Data di pubblicazione: 2018-02-16
Rated 5 di 5 di da Good antibody This antibody shows great reactivity. It is highly specific
Data di pubblicazione: 2017-09-19
Rated 5 di 5 di da Western Blot We used the HSP 47 antibody for Western Blot, the stripes are very fine at 47kda.
Data di pubblicazione: 2017-05-31
Rated 5 di 5 di da strong recommend antibody We observed clear band when diluted 1:500 ,Great help to us, strongly recommended.
Data di pubblicazione: 2017-05-28
Rated 5 di 5 di da works very good We use it usually , works very well in WB at 1:500 dilution.
Data di pubblicazione: 2017-02-26
Rated 5 di 5 di da A strong HSP 47 monoclonal choice! This excellent antibody has great reactivity in a wide range of applications. It demonstrates strong bands in a Western blot and has provide nice immunofluoresence and Immunoperoxidase staining . This product is well characterized in the primary literature.
Data di pubblicazione: 2016-12-20
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