GGCX CRISPR/Cas9 KO Plasmid (h): sc-407037
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GGCX CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GGCX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GGCX CRISPR/Cas9 KO Plasmid (h) | sc-407037 | 20 µg | $397.00 |
Overview
GGCX (gamma-glutamyl carboxylase) is an endoplasmic reticulum membrane enzyme that catalyzes vitamin K–dependent γ-carboxylation of glutamate residues, generating Gla domains required for calcium binding. This post-translational modification is essential for maturation and activity of multiple secreted proteins, including coagulation factors and other vitamin K–dependent proteins, linking GGCX to hemostasis, extracellular protein function, and redox cycling within the vitamin K pathway. GGCX activity intersects with ER protein processing and secretory pathway quality control because proper carboxylation influences protein folding and secretion efficiency. Genetic disruption or altered function of GGCX has been associated with bleeding phenotypes and broader vitamin K–dependent protein dysfunction, making it a useful target for studying coagulation-related biology and ER-linked post-translational regulation.
GGCX CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GGCX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GGCX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GGCX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GGCX protein expression.
This CRISPR knockout system enables efficient generation of GGCX-deficient cell models for investigation of GGCX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GGCX exon(s) critical for GGCX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GGCX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GGCX CRISPR/Cas9 KO Plasmid (h) and GGCX CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GGCX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GGCX HDR Plasmid (h) and GGCX HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GGCX homology arms to support homology-directed repair at defined GGCX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.