GATA-2 Antibody (CG2-96): sc-267

GATA-2 Antibody (CG2-96) is a mouse monoclonal IgG1 kappa light chain antibody that detects GATA-2 protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). GATA-2 monoclonal antibody (CG2-96) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. GATA-2 plays a crucial role in hematopoiesis and is essential for the development of multiple blood lineages, including erythroid and myeloid cells. GATA-2 expression is particularly prominent in embryonic brain and liver tissues, as well as in endothelial and HeLa cells. GATA-2 contains a highly conserved zinc finger DNA-binding domain, which allows binding to specific DNA sequences, thereby regulating gene expression. GATA-2 has been shown to interact with other GATA family members, such as GATA-1 and GATA-3, which enhances regulatory capabilities. This interaction suggests that GATA-2 can modulate the expression of genes involved in cell differentiation and development, making GATA-2 a key player in various biological processes. Understanding GATA-2 function and interactions is vital for elucidating GATA-2′s role in normal physiology and implications in diseases such as leukemia and other hematological disorders.

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GATA-2 Antibody (CG2-96) References:

1. Roles of hematopoietic transcription factors GATA-1 and GATA-2 in the development of red blood cell lineage.  |  Ohneda, K. and Yamamoto, M. 2002. Acta Haematol. 108: 237-45. PMID: 12432220
2. Human transcription factor GATA-2. Evidence for regulation of preproendothelin-1 gene expression in endothelial cells.  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
3. The paralogous hematopoietic regulators Lyl1 and Scl are coregulated by Ets and GATA factors, but Lyl1 cannot rescue the early Scl-/- phenotype.  |  Chan, WY., et al. 2007. Blood. 109: 1908-16. PMID: 17053063
4. Cytokine and integrin stimulation synergize to promote higher levels of GATA-2, c-myb, and CD34 protein in primary human hematopoietic progenitors from bone marrow.  |  Dao, MA. and Nolta, JA. 2007. Blood. 109: 2373-9. PMID: 17095623
5. Context-dependent GATA factor function: combinatorial requirements for transcriptional control in hematopoietic and endothelial cells.  |  Wozniak, RJ., et al. 2007. J Biol Chem. 282: 14665-74. PMID: 17347142
6. A Gata2 intronic enhancer confers its pan-endothelia-specific regulation.  |  Khandekar, M., et al. 2007. Development. 134: 1703-12. PMID: 17395646
7. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer.  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
8. GATA-2 mediated regulation of normal hematopoietic stem/progenitor cell function, myelodysplasia and myeloid leukemia.  |  Rodrigues, NP., et al. 2012. Int J Biochem Cell Biol. 44: 457-60. PMID: 22192845
9. GATA-2 inhibits transforming growth factor-β signaling pathway through interaction with Smad4.  |  Dong, XM., et al. 2014. Cell Signal. 26: 1089-97. PMID: 24509415
10. GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut.  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
11. DNA-binding specificities of the GATA transcription factor family.  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
12. The human GATA-6 gene: structure, chromosomal location, and regulation of expression by tissue-specific and mitogen-responsive signals.  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GATA-2 Antibody (CG2-96) has 74 citations in a variety of scientific publications.

Primary antibodies like Anti-GATA-2 Antibody (CG2-96) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GATA-2 Antibody (CG2-96) Specifications

Alternate Product Name: GATA2

Product Clone Name: CG2-96

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GATA-2

Epitope Species:

Product Reactivity/Detection: GATA-2

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for GATA-2 Antibody (CG2-96): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred GATA-2 antibody is GATA-2 Antibody (H-6): sc-515178.