FKSG80 CRISPR/Cas9 KO Plasmid (m): sc-433960
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FKSG80 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FKSG80 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKSG80 CRISPR/Cas9 KO Plasmid (m) | sc-433960 | 20 µg | $397.00 |
Overview
Hcar1 encodes a lactate-sensing G protein–coupled receptor implicated in coupling extracellular lactate availability to intracellular signaling programs that regulate cellular metabolism and immune modulation in mouse tissues. Receptor activation is linked to Gi/o-dependent suppression of cAMP and downstream effects on MAPK signaling, transcriptional responses, and metabolic rewiring during hypoxia or high glycolytic flux. Through its role in lactate signaling, Hcar1 is studied in contexts of inflammation, neurobiology, adipose and muscle physiology, and tumor-associated microenvironments where lactate gradients shape cell behavior. Dysregulated lactate receptor signaling is therefore relevant to pathways that coordinate energy homeostasis and stress adaptation across multiple organ systems.
FKSG80 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hcar1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hcar1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hcar1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FKSG80 protein expression.
This CRISPR knockout system enables efficient generation of Hcar1-deficient cell models for investigation of FKSG80 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hcar1 exon(s) critical for FKSG80 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hcar1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FKSG80 CRISPR/Cas9 KO Plasmid (m) and FKSG80 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hcar1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FKSG80 HDR Plasmid (m) and FKSG80 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hcar1 homology arms to support homology-directed repair at defined Hcar1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.