CRSP130 CRISPR/Cas9 KO Plasmid (h): sc-406593
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRSP130 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRSP130 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRSP130 CRISPR/Cas9 KO Plasmid (h) | sc-406593 | 20 µg | $397.00 |
Overview
MED23 encodes CRSP130, a core subunit of the Mediator complex that couples sequence-specific transcription factors to RNA polymerase II, coordinating transcription initiation and signal-dependent gene expression programs. CRSP130 helps integrate regulatory inputs from developmental and stress-responsive pathways to modulate promoter activity and chromatin-associated transcriptional control. Through Mediator-dependent regulation of broad gene networks, MED23 has been linked in the literature to altered cell growth and differentiation states and to dysregulated transcriptional outputs observed across multiple disease contexts. Its central role in transcriptional circuitry makes MED23 a useful target for dissecting pathway-to-promoter communication and transcription factor dependency in human cell models.
CRSP130 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MED23 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MED23 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MED23 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRSP130 protein expression.
This CRISPR knockout system enables efficient generation of MED23-deficient cell models for investigation of CRSP130 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MED23 exon(s) critical for CRSP130 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MED23 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRSP130 CRISPR/Cas9 KO Plasmid (h) and CRSP130 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MED23 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRSP130 HDR Plasmid (h) and CRSP130 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MED23 homology arms to support homology-directed repair at defined MED23 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.