CHSY3 CRISPR/Cas9 KO Plasmid (m): sc-429749
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHSY3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHSY3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHSY3 CRISPR/Cas9 KO Plasmid (m) | sc-429749 | 20 µg | $397.00 |
Overview
Chsy3 encodes chondroitin sulfate synthase 3 (CHSY3), a Golgi-resident glycosyltransferase that participates in the elongation of chondroitin sulfate glycosaminoglycan chains on proteoglycans. Through coordination with other chondroitin polymerases and sulfotransferases, CHSY3 influences extracellular matrix organization, growth factor sequestration, and cell–matrix signaling that shape tissue morphogenesis and homeostasis. In mouse systems, altered chondroitin sulfate composition can affect cartilage and skeletal development, neural plasticity, and inflammatory microenvironments by modulating proteoglycan-dependent pathways. As a result, Chsy3 is relevant to mechanistic studies of extracellular matrix remodeling and proteoglycan biology in models of musculoskeletal and neurobiological phenotypes.
CHSY3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Chsy3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Chsy3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Chsy3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHSY3 protein expression.
This CRISPR knockout system enables efficient generation of Chsy3-deficient cell models for investigation of CHSY3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Chsy3 exon(s) critical for CHSY3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Chsy3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHSY3 CRISPR/Cas9 KO Plasmid (m) and CHSY3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Chsy3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHSY3 HDR Plasmid (m) and CHSY3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Chsy3 homology arms to support homology-directed repair at defined Chsy3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.