Date published: 2025-9-10

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Anticorpo CD81 (5A6): sc-23962

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Schede Tecniche
  • CD81 Antibody 5A6 è un monoclonale di topo IgG1 κ, citato in 127 pubblicazioni, fornito in 200 µg/ml
  • raccolto contro cellule OCI-LY8
  • CD81 Antibody (5A6) é raccomandato per il rilevamento di CD81 di origine mouse, rat e human in WB, IP, IF e FCM
  • CD81 Antibody (5A6) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
  • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
  • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
  • Contattaci per ricevere un FREE 10 µg sample di CD81 (5A6): sc-23962.
  • m-IgG Fc BP-HRP e 1 BP-HRP">m-IgG1 BP-HRP sono i reagenti secondari preferiti per l'anticorpo CD81 (5A6) for WB applications. Questi reagenti sono ora offerti in bundle con l'anticorpo CD81 (5A6)(vedere le informazioni per l'ordine sotto).

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L'anticorpo CD81 (5A6) è un anticorpo monoclonale di topo IgG1 a catena leggera kappa specificamente progettato per colpire la proteina CD81, nota anche come TAPA-1, un membro importante della famiglia delle tetraspanine, caratterizzato da quattro domini transmembrana. Il CD81 è ampiamente espresso nelle cellule di origine ematopoietica, neuroectodermica e mesenchimale, dove svolge un ruolo vitale nella crescita cellulare, nella trasduzione del segnale e nell'organizzazione dei microdomini di membrana. Il CD81 subisce modifiche post-traduzionali essenziali, tra cui la palmitoilazione e la glicosilazione, che sono cruciali per la corretta localizzazione, la stabilità e le interazioni con altre proteine di membrana come CD37, CD53, CD19, CD21 e gli antigeni MHC di classe II. L'anticorpo anti-CD81 (5A6) è stato sviluppato utilizzando la linea cellulare di linfoma B OCI-LY8, assicurando un'efficace rilevazione del CD81 nelle specie di topo, ratto e uomo attraverso applicazioni come il western blotting (WB), l'immunoprecipitazione (IP), l'immunofluorescenza (IF) e la citometria a flusso (FCM). Disponibile sia in forme coniugate, tra cui perossidasi di rafano (HRP), ficoeritrina (PE), isotiocianato di fluoresceina (FITC) e vari coniugati Alexa Fluor®, sia in forme non coniugate, l'anticorpo monoclonale CD81 (5A6) offre opzioni versatili per diverse esigenze sperimentali. Prendendo di mira il CD81, i ricercatori possono studiare processi critici come l'attivazione delle cellule immunitarie, i meccanismi di ingresso virale e le complesse vie di segnalazione che regolano le funzioni cellulari. Le modifiche post-traduzionali del CD81 sono particolarmente importanti per comprendere il ruolo del CD81 in condizioni patologiche come la metastasi del cancro e i disturbi immunitari, rendendo l'anticorpo anti-CD81 (5A6) uno strumento essenziale per la ricerca di base e applicata.

Solo per ricerca in vitro. Non destinato ad uso Diagnostico o Terapeutico.

Alexa Fluor® è un marchio registrato di Molecular Probes Inc., OR., USA

LI-COR® e Odyssey® sono marchi registrati di LI-COR Biosciences

Informazioni ordini

Nome del prodottoCodice del prodottoUNITÀPrezzoQuantitàPreferiti

CD81 Anticorpo (5A6)

sc-23962
200 µg/ml
$316.00

CD81 (5A6): m-IgG Fc BP-HRP Bundle

sc-526643
200 µg Ab; 10 µg BP
$354.00

CD81 (5A6): m-IgG1 BP-HRP Bundle

sc-532016
200 µg Ab; 20 µg BP
$354.00

CD81 Anticorpo (5A6) AC

sc-23962 AC
500 µg/ml, 25% agarose
$416.00

CD81 Anticorpo (5A6) HRP

sc-23962 HRP
200 µg/ml
$316.00

CD81 Anticorpo (5A6) FITC

sc-23962 FITC
200 µg/ml
$330.00

CD81 Anticorpo (5A6) PE

sc-23962 PE
200 µg/ml
$343.00

CD81 Anticorpo (5A6) Alexa Fluor® 488

sc-23962 AF488
200 µg/ml
$357.00

CD81 Anticorpo (5A6) Alexa Fluor® 546

sc-23962 AF546
200 µg/ml
$357.00

CD81 Anticorpo (5A6) Alexa Fluor® 594

sc-23962 AF594
200 µg/ml
$357.00

CD81 Anticorpo (5A6) Alexa Fluor® 647

sc-23962 AF647
200 µg/ml
$357.00

CD81 Anticorpo (5A6) Alexa Fluor® 680

sc-23962 AF680
200 µg/ml
$357.00

CD81 Anticorpo (5A6) Alexa Fluor® 790

sc-23962 AF790
200 µg/ml
$357.00

What is the isoelectric point for CD-81 antibody?

Richiesta da: Sumita
Thank you for your question. We do not characterize the isoelectric point of our antibodies.
Risposta da: BlakeJ
Data di pubblicazione: 2025-07-23

Hello, I have used the CD81 antibody for western blot at 1:1500 in 5% MILK on exosomes from human plasma. Do you know why I get two bands, one at 23 kDa and another one at 48 kDa? Many thanks Juliana

Richiesta da: Juliana Redondo
Thank you for your question. The expected molecular weight for CD81 is ~ 22-26 kDa. We have not tested this antibody specifically in human plasma, and have not observed a higher molecular weight signal at 48 kDa in the cell lines tested. CD81 has been reported to dimerize (PMID: 20375010) and that may be a consideration. We would also recommend including a secondary antibody control, where you omit the primary antibody from the incubation, to confirm if the higher molecular weight band might be due to non-specific binding of the secondary antibody alone.
Risposta da: Technical Support
Data di pubblicazione: 2017-10-16

Hi, I have used this antibody at 1:100 concentration on a western blot (10% SDS-PAGE gel) loading some exosomal protein. Could you explain please why I get not only the 26 kDa band, but also a band at about 50 kDa? Thank you.

Richiesta da: VivSal
Thank you for your question. We have not tested this antibody on exosomal protein, or observed a 50 kDa band in Western blot. We would recommend including a secondary antibody control (omit primary antibody) to identify if the 50 kDa band may be a result of non-specific binding of the secondary antibody alone. It could also be your secondary antibody binding to heavy chain IgG in your samples. Below is a link to our recommended protocol for Western blotting. Please contact our Technical Service Department if for additional support with troubleshooting the antibody. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-10-16

I have tried using this antibody on a western blot (after running an SDS-PAGE gel) with a homemade K562 lysate as control and did not get a signal at 1:100 dilution. I tried it 3 times previously on other cell lines at 1:200 w/no success. Any suggestions?

Richiesta da: Tali
Thank you for your question. If you have no signal, we would recommend verifying that your anti-mouse secondary is working well. We would also recommend including a loading control antibody (e.g., Actin or GAPDH) to ensure that your transfer conditions are good, as well as your signal detection conditions (e.g., chemiluminescence or other). We have successfully used this antibody for WB at a dilution of 1:200, loading 50 µg of K-562 whole cell lysate. A link to our complete recommended protocol is provided below. If you continue to have negative results, please contact our Technical Service Department so we can provide further troubleshooting support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting .
Risposta da: Technical Support
Data di pubblicazione: 2017-04-26

What concentration would you recommend using SC-20048 at, to detect a protein in paraffin-embedded human tissue? We have used 1:1000 successfully for western blots. Please can you reply at your earliest convenience.

Richiesta da: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. This is a starting dilution, so it is possible that you can use it much more diluted! We also recommend performing an antigen retrieval step, ideally sodium citrate with heat. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Risposta da: Tech Service
Data di pubblicazione: 2017-02-22

1, What concentration do you recommend using SC-23962 on paraffin embedded human tissue sections? We have this antibody, so would like to try. ; 2. Do you think that SC-23962 FITC would work better and at what conc. for same application, ie IHC(P)?

Richiesta da: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. We do recommend performing an antigen retrieval step. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining You can use the FITC conjugated form, although we do not generally use FITC conjugated antibodies for IHC in house, so I do not have test results for this. If you wish to try it, I would stick with the 1:50 starting dilution as well. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Risposta da: Tech Service
Data di pubblicazione: 2017-02-22

Can I use this antibody for staining of mouse tissue, since it is a mouse antibody?

Richiesta da: Anna99
Yes. CD81 Antibody (5A6): sc-23962 is available conjugated to either phycoerythrin (sc-23962 PE) or fluoroscein (sc-23962 FITC), 200 µg/ml, for IF, IHC(P) and FCM.
Risposta da: Tech Service 5
Data di pubblicazione: 2017-01-05
  • y_2025, m_9, d_6, h_5CST
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Rated 4 di 5 di da Great antibodyI request a sample of the antibody and it worked well!
Data di pubblicazione: 2023-07-31
Rated 4 di 5 di da buon funzionamentoHo effettuato un solo esperimento per verificare la reattività nel cane e ha funzionato
Data di pubblicazione: 2023-07-14
Rated 1 di 5 di da WBでバンドが検出できませんでした。エクソソームサンプルでCD81のバンドが全く検出できませんでした。同じサンプルで他のCD81抗体を使うとCD81を検出できたのでサンプルは問題ないと考えています。
Data di pubblicazione: 2021-09-09
Rated 2 di 5 di da No band in Western blotI cannot see any band in Western blot after a nice transfer (all MW completely transfered; blotting using 1:200 of this antibody and 2nd antibody sc-516102 at 1:1000) of exosomes isolated by ultracentrifugation and further separated by density centrifugation. Perhaps I have low concentration of exosomes, but I expected to get at least a faint band.
Data di pubblicazione: 2021-05-25
Rated 5 di 5 di da Works well in western blotThe antibody detected CD81 in Mesenchymal stem cells at the expected molecular size.
Data di pubblicazione: 2020-09-18
Rated 5 di 5 di da Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
Data di pubblicazione: 2018-11-19
Rated 5 di 5 di da Excellent antibody for ICC stainingThis antibody is gorgeous for ICC staining, please see the attached confocal image. Highly recommended. I tried 1:50 dilution for the first time, and you may further dilute it to 1:100 or even 1:200 to decrease the background a little bit, and it should work even better. I feel the overall quality of SCBT antibodies is getting much better,
Data di pubblicazione: 2018-11-08
Rated 5 di 5 di da likeMembrane localization observed by Immunofluorescence staining of methanol-fixed embedded HeLa cells, also good for staining.
Data di pubblicazione: 2017-05-07
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Anticorpo CD81 (5A6) valutazione 4.1 di 5 di 14.
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