Calnexin Double Nickase Plasmid (m): sc-419436-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calnexin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Calnexin Double Nickase Plasmid (m) and Calnexin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Canx. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calnexin Antibody (AF18): sc-23954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calnexin Double Nickase Plasmid (m) | sc-419436-NIC | 20 µg | $410.00 | |||
Calnexin Double Nickase Plasmid (m2) | sc-419436-NIC-2 | 20 µg | $410.00 |
Mouse Canx encodes calnexin, an ER-resident lectin chaperone that binds monoglucosylated N-linked glycans to promote folding and quality control of nascent glycoproteins. Calnexin functions within the calnexin/calreticulin cycle in coordination with ERp57 and related oxidoreductases, linking glycoprotein maturation to ER-associated degradation (ERAD) and the unfolded protein response (UPR). By regulating trafficking and stability of membrane and secreted proteins, calnexin influences proteostasis, oxidative stress handling, and immune-related receptor biogenesis. Dysregulated calnexin-dependent quality control has been associated with ER stress phenotypes and altered signaling outputs relevant to neurodegeneration, metabolic dysfunction, and inflammatory pathways in experimental models.
Calnexin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Canx locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Canx. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Canx function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Canx-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.