Cacna2d2 Antibody (G-5): sc-365911

Cacna2d2 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cacna2d2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Cacna2d2 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The CACNA2D2 gene encodes the calcium channel, voltage-dependent alpha2delta-2 protein, which serves as a crucial regulatory subunit of voltage-dependent calcium channels. Cacna2d2 is essential for mediating calcium influx into cells, a process vital for numerous physiological functions, including neurotransmitter release and muscle contraction. Cacna2d2 interacts with alpha-1, beta, and gamma subunits in a precise 1:1:1:1 ratio to form a functional calcium channel, with expression predominantly observed in the brain and heart, highlighting Cacna2d2′s importance in central nervous system function. Disruptions in the CACNA2D2 gene have been implicated in various neurological disorders, such as cerebellar ataxias and epilepsy, while Cacna2d2 expression is notably reduced in several cancers, including lung and breast cancer, indicating a potential role as a tumor suppressor. The CACNA2D2 gene is located in the tumor suppressor region of chromosome 3p21.3 in humans, and Cacna2d2 shares 56% amino acid homology with the alpha2/delta-1 subunit, although these proteins exhibit distinct tissue expression patterns, underscoring the complexity of calcium signaling pathways in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cacna2d2 Antibody (G-5) References:

1. Differential effect of gabapentin on neuronal and muscle calcium currents.  |  Alden, KJ. and García, J. 2001. J Pharmacol Exp Ther. 297: 727-35. PMID: 11303064
2. Ducky mouse phenotype of epilepsy and ataxia is associated with mutations in the Cacna2d2 gene and decreased calcium channel current in cerebellar Purkinje cells.  |  Barclay, J., et al. 2001. J Neurosci. 21: 6095-104. PMID: 11487633
3. The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha 2 delta-2 protein with abnormal function.  |  Brodbeck, J., et al. 2002. J Biol Chem. 277: 7684-93. PMID: 11756448
4. Expression of several genes in the human chromosome 3p21.3 homozygous deletion region by an adenovirus vector results in tumor suppressor activities in vitro and in vivo.  |  Ji, L., et al. 2002. Cancer Res. 62: 2715-20. PMID: 11980673
5. CACNA2D2-mediated apoptosis in NSCLC cells is associated with alterations of the intracellular calcium signaling and disruption of mitochondria membrane integrity.  |  Carboni, GL., et al. 2003. Oncogene. 22: 615-26. PMID: 12555074
6. [New tumor suppressor genes in hot spots of human chromosome 3: new methods of identification].  |  Braga, EA., et al. 2003. Mol Biol (Mosk). 37: 194-211. PMID: 12723467
7. RASSF1A is a target tumor suppressor from 3p21.3 in nasopharyngeal carcinoma.  |  Chow, LS., et al. 2004. Int J Cancer. 109: 839-47. PMID: 15027117
8. Discovery of frequent homozygous deletions in chromosome 3p21.3 LUCA and AP20 regions in renal, lung and breast carcinomas.  |  Senchenko, VN., et al. 2004. Oncogene. 23: 5719-28. PMID: 15208675
9. The biology and pharmacology of calcium channel alpha2-delta proteins Pfizer Satellite Symposium to the 2003 Society for Neuroscience Meeting. Sheraton New Orleans Hotel, New Orleans, LA November 10, 2003.  |  Taylor, CP. 2004. CNS Drug Rev. 10: 183-8. PMID: 15317151
10. Cerebellar ataxia, seizures, premature death, and cardiac abnormalities in mice with targeted disruption of the Cacna2d2 gene.  |  Ivanov, SV., et al. 2004. Am J Pathol. 165: 1007-18. PMID: 15331424
11. Biallelic CACNA2D2 variants in epileptic encephalopathy and cerebellar atrophy.  |  Punetha, J., et al. 2019. Ann Clin Transl Neurol. 6: 1395-1406. PMID: 31402629
12. Altered Hippocampal Activation in Seizure-Prone CACNA2D2 Knock-out Mice.  |  Danis, AB., et al. 2024. eNeuro. 11: PMID: 38749701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cacna2d2 Antibody (G-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Cacna2d2 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cacna2d2 Antibody (G-5) Specifications

Alternate Product Name: Cacna2d2

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-210

Epitope Target: Cacna2d2

Epitope Species: human

Product Reactivity/Detection: Cacna2d2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Cacna2d2 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin