C/EBP δ CRISPR/Cas9 KO Plasmid (h): sc-400772

Overview

CEBPD encodes the transcription factor C/EBPδ, a basic leucine zipper protein that regulates gene expression programs linked to innate immune signaling, cellular stress responses, and differentiation. C/EBPδ is induced by inflammatory cues and participates in transcriptional networks downstream of cytokine and Toll-like receptor pathways, shaping expression of mediators involved in acute-phase and myeloid-associated functions. It also interfaces with cell-cycle control and survival pathways, contributing to context-dependent regulation of proliferation, senescence, and metabolic adaptation. Dysregulated CEBPD activity has been reported across inflammatory conditions and multiple cancer contexts, supporting its use as a mechanistic node for studying transcriptional control in disease-relevant signaling states.

C/EBP δ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CEBPD gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CEBPD together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CEBPD open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C/EBP δ protein expression.

This CRISPR knockout system enables efficient generation of CEBPD-deficient cell models for investigation of C/EBP δ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting CEBPD exon(s) critical for C/EBP δ function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple CEBPD genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by C/EBP δ CRISPR/Cas9 KO Plasmid (h) and C/EBP δ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CEBPD locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by C/EBP δ HDR Plasmid (h) and C/EBP δ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CEBPD homology arms to support homology-directed repair at defined CEBPD target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.