Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ALX4 CRISPR/Cas9 KO Plasmid (m): sc-419095
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALX4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ALX4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ALX4 Antibody (KAB4): sc-33643
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALX4 CRISPR/Cas9 KO Plasmid (m) | sc-419095 | 20 µg | $397.00 |
Overview
Alx4 encodes the ALX4 homeobox transcription factor, a nuclear regulator of developmental gene expression programs that shape craniofacial patterning, limb and skeletal morphogenesis, and mesenchymal differentiation. ALX4 influences transcriptional networks controlling proliferation and positional identity, intersecting with pathways involved in osteogenic lineage commitment and tissue boundary formation. In mouse models, altered Alx4 function is associated with defects in skull vault development, craniofacial malformations, and digit patterning abnormalities, supporting its utility as a node for studying congenital morphogenesis. These properties make ALX4 a relevant target for investigating gene regulatory circuits underlying skeletal development and related developmental phenotypes.
ALX4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Alx4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Alx4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Alx4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ALX4 protein expression.
This CRISPR knockout system enables efficient generation of Alx4-deficient cell models for investigation of ALX4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Alx4 exon(s) critical for ALX4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Alx4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ALX4 CRISPR/Cas9 KO Plasmid (m) and ALX4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Alx4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ALX4 HDR Plasmid (m) and ALX4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Alx4 homology arms to support homology-directed repair at defined Alx4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.