Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
alpha Synuclein Double Nickase Plasmid (m): sc-423051-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- alpha Synuclein Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- alpha Synuclein Double Nickase Plasmid (m) and alpha Synuclein Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Snca. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: alpha Synuclein Antibody (2B2D1): sc-53955
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
alpha Synuclein Double Nickase Plasmid (m) | sc-423051-NIC | 20 µg | $410.00 |
Snca encodes mouse alpha-synuclein, a presynaptic protein implicated in synaptic vesicle trafficking, SNARE complex assembly, and regulation of neurotransmitter release. It associates with lipid membranes and can influence vesicle pool dynamics, endocytosis, and proteostasis pathways including ubiquitin–proteasome and autophagy-lysosome systems. Altered alpha-synuclein homeostasis and aggregation are central to mechanisms of neurodegeneration, linking Snca to neuronal stress responses, mitochondrial dysfunction, and inflammatory signaling. Snca is therefore widely used to model molecular events relevant to synucleinopathies and to interrogate neuronal and glial pathways that shape protein misfolding and clearance.
alpha Synuclein Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Snca locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Snca. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Snca function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Snca-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.