Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Agrin Double Nickase Plasmid (h): sc-401256-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Agrin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Agrin Double Nickase Plasmid (h) and Agrin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AGRN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Agrin Antibody (D-2): sc-374117
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Agrin Double Nickase Plasmid (h) | sc-401256-NIC | 20 µg | $410.00 | |||
Agrin Double Nickase Plasmid (h2) | sc-401256-NIC-2 | 20 µg | $410.00 |
AGRN encodes agrin, a large heparan sulfate proteoglycan of the extracellular matrix that organizes cell–matrix interactions and regulates synapse formation and stability. In neuromuscular and central synapses, agrin signaling coordinates clustering and maintenance of postsynaptic specializations, supporting pathways that control receptor localization, cytoskeletal remodeling, and membrane domain organization. Beyond synaptogenesis, agrin contributes to basement membrane architecture, mechanotransduction, and neuronal and glial communication. Dysregulated AGRN expression or agrin processing has been associated with altered synaptic integrity and tissue remodeling in neuromuscular and neurodegenerative contexts, supporting its use as a target in functional genomics studies of connectivity and extracellular matrix signaling.
Agrin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AGRN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AGRN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AGRN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AGRN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.