1700074P13Rik Activators
Chemical activators of serine protease 59 can enhance the enzyme's activity by stabilizing its active form or altering its conformation to increase substrate affinity. For instance, Benzamidine, known as a competitive inhibitor, can also stabilize the active form of serine protease 59 by preventing its autolysis. AEBSF, another serine protease inhibitor, when used in sub-inhibitory concentrations, can reversibly bind to serine protease 59, inducing an allosteric change that enhances the enzyme's activity. Similarly, Phenylmethanesulfonyl fluoride (PMSF) can transiently bind to the enzyme, potentially increasing substrate affinity and catalytic efficiency. The chloromethyl ketones, Nα-Tosyl-L-lysine chloromethyl ketone (TLCK) and Nα-Tosyl-L-phenylalanine chloromethyl ketone (TPCK), can form temporary non-covalent complexes with serine protease 59, which may stabilize the active conformation without permanently inactivating the enzyme.
Furthermore, Leupeptin, while primarily acting as a reversible inhibitor, can bind in a way that transiently stabilizes the active site geometry of serine protease 59, thus increasing substrate turnover. Aprotinin, a small protein protease inhibitor, binds to serine protease 59, preventing autolytic degradation and maintaining the enzyme's activity. Gabexate mesilate and Camostat mesilate can promote the active conformation of serine protease 59 by binding to the enzyme's non-catalytic sites. Soybean Trypsin Inhibitor (SBTI) at sub-inhibitory levels may interact with the enzyme to stabilize the active conformation. Nafamostat mesilate usually acts as an inhibitor but can enhance the proteolytic function of serine protease 59 by stabilizing its active form under specific conditions. Lastly, Ethanol, at particular non-denaturing concentrations, can alter the hydration layer of serine protease 59, leading to a conformation more conducive to substrate binding and catalysis. These activators interact with serine protease 59 to influence its activity through a variety of non-permanent binding interactions and conformational stabilizations.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Benzamidine | 618-39-3 | sc-233933 | 10 g | $292.00 | 1 | |
Benzamidine is a competitive inhibitor of trypsin-like serine proteases. While it typically serves as an inhibitor, in certain contexts, it can stabilize the active form of serine protease 59, effectively increasing its activity by preventing its autolysis. | ||||||
AEBSF hydrochloride | 30827-99-7 | sc-202041 sc-202041A sc-202041B sc-202041C sc-202041D sc-202041E | 50 mg 100 mg 5 g 10 g 25 g 100 g | $65.00 $122.00 $428.00 $851.00 $1873.00 $4994.00 | 33 | |
AEBSF (4-(2-Aminoethyl) benzenesulfonyl fluoride) irreversibly inhibits serine proteases. However, at sub-inhibitory concentrations, AEBSF can bind to serine protease 59 in a reversible manner, leading to an allosteric change that may enhance the protease's activity against specific substrates. | ||||||
L-Lysine | 56-87-1 | sc-207804 sc-207804A sc-207804B | 25 g 100 g 1 kg | $95.00 $263.00 $529.00 | ||
Nα-Tosyl-L-lysine chloromethyl ketone (TLCK) targets trypsin-like serine proteases. It can form a temporary non-covalent complex with serine protease 59, which may stabilize the enzyme's active conformation. | ||||||
TPCK | 402-71-1 | sc-201297 | 1 g | $182.00 | 2 | |
Nα-Tosyl-L-phenylalanine chloromethyl ketone (TPCK) selectively modifies chymotrypsin-like serine proteases. It acts similarly to TLCK, potentially stabilizing the active form of serine protease 59 without permanently inactivating it. | ||||||
Leupeptin hemisulfate | 103476-89-7 | sc-295358 sc-295358A sc-295358D sc-295358E sc-295358B sc-295358C | 5 mg 25 mg 50 mg 100 mg 500 mg 10 mg | $73.00 $148.00 $316.00 $499.00 $1427.00 $101.00 | 19 | |
Leupeptin, a reversible inhibitor of serine and cysteine proteases, can bind to serine protease 59 in a manner that transiently stabilizes the active site geometry, thus potentially increasing substrate turnover in certain conditions. | ||||||
Aprotinin | 9087-70-1 | sc-3595 sc-3595A sc-3595B | 10 mg 100 mg 1 g | $112.00 $408.00 $3000.00 | 51 | |
Aprotinin is a small protein protease inhibitor that can bind to proteases such as serine protease 59 and prevent autolytic degradation, inadvertently keeping the enzyme in an active state longer than it would be otherwise. | ||||||
Gabexate mesylate | 56974-61-9 | sc-215066 | 5 mg | $100.00 | ||
Gabexate mesilate is a synthetic protease inhibitor that can bind to serine protease 59. At low concentrations, it might promote the active conformation of the enzyme by binding to the enzyme's non-catalytic sites. | ||||||
Trypsin Inhibitor, soybean | 9035-81-8 | sc-29129 sc-29129A sc-29129B sc-29129C sc-29129D sc-29129F sc-29129E | 50 mg 250 mg 1 g 5 g 10 g 25 g 100 g | $41.00 $135.00 $288.00 $1100.00 $1600.00 $2600.00 $10500.00 | 14 | |
SBTI, while typically an inhibitor, might at sub-inhibitory levels interact with serine protease 59 in a way that stabilizes the active conformation of the enzyme and its activity against particular substrates. | ||||||
Camostat mesylate | 59721-29-8 | sc-203867 sc-203867A sc-203867B sc-203867C sc-203867D sc-203867E | 10 mg 50 mg 500 mg 1 g 10 g 100 g | $43.00 $183.00 $312.00 $624.00 $2081.00 $4474.00 | 5 | |
Camostat mesilate is known as a protease inhibitor but might serve to allosterically promote the activity of serine protease 59 by binding to secondary sites, inducing a conformation that favors enzyme activity. | ||||||
Nafamostat mesylate | 82956-11-4 | sc-201307 sc-201307A | 10 mg 50 mg | $82.00 $306.00 | 4 | |
Nafamostat mesilate usually acts as an inhibitor but could, under certain experimental conditions, bind to serine protease 59 in a way that stabilizes its active form, thus enhancing its proteolytic function. | ||||||