CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.
CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.
Cre Vector Productos CRISPR Knockout y Activación (): sc-418923
20 µg de plásmido de ADN purificado, listo para usar; Indicado para 20 trasnfecciones
El Vercto Cre expresa una recombinasa Cre, para catalizar la recombinación especifica de sitio del ADN entre los dos LoxP
El vector Cre está diseñado para dirigirse específicamente a los sitios LoxP que flanquean los marcadores de selección insertados por el plásmido HDR y eliminar el material genético.
El Vercto Cre contiene un promotor CMV para dirigir la expresion de la recombinasa Cre
Tras la transfección, las células pueden analizarse mediante WB, IF ó IHC utilizando anticuerpos contra el gen diana ó transfectando de nuevo para edición génica adicional
For assistance please contact Technical service . Please call 800-457-3801 or email scbt@scbt.com.
Respondida por: BlakeJ
Fecha de publicación: 2026-01-21
Hi, can the above plasmid be used for yeast namely saccharomyces cerevisiae?
Preguntado por: NT777
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Respondida por: lai13
Fecha de publicación: 2025-03-03
How to transfect the cre-vector to the cells that already have puromycin gene incorporated by HDR vector? transfect, then wait two or three days and consider those cells puromycin free? correct? do I select?
Preguntado por: michaela
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Respondida por: lai13
Fecha de publicación: 2024-07-25
Can you provide the plasmid map and size of the vector?
Thanks,
Preguntado por: Chris1
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Respondida por: lai13
Fecha de publicación: 2024-07-25
Does this kit includes a vector to flanked my target gene?
Preguntado por: Ldas01
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Respondida por: Blake J
Fecha de publicación: 2022-01-26
How large is this plasmid?
Preguntado por: Dkr0
Thank you for your question. Our Cre Vector sc-418923 has a size of approximately 5.9 kilobases.
Respondida por: Tech Support Europe
Fecha de publicación: 2024-04-20
Why is CRE Vector Transfection "Recommended" after for CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection (following selection)? Is it simply to remove the selection marker? Or, does it help in any way with knocking out the gene?
Preguntado por: jmd6207
Thank you for your question. It is not mandatory, and only recommended if you wish to removed the puromycin selection marker. Removing the HDR plasmid insert using the CRE vector does not help with knocking out the gene. It is useful if you wish you knock out a second gene or if you need you cells to no longer be puromycin resistant.
Respondida por: Tech Service
Fecha de publicación: 2024-03-19
whats the cre loxp systems
Preguntado por: waleed xel
Thank you for your question. The Cre-Lox system is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. You can find our more about the Cre-Lox system at any of the links below:
https://en.wikipedia.org/wiki/Cre-Lox_recombination
https://www.youtube.com/watch?v=oLPjiwM0G7A&app=desktop
Rated 5 de
5 por
Jelena de
Very useful product.Very useful tool to achieve double or triple knock-out with the CRISPR system since the most of Santa Cruz CRISPR products are resistant to the same antibiotic-pyromycin.
Fecha de publicación: 2017-04-25
Rated 4 de
5 por
mask de
Remove the marker gene efficientlyI used this vector to remove the neo marker-gene in the pig primary cells . The marker-free efficiency was so high to 80% .I nucleofected the 4ug Cre vector to the 1000000 cells by ALONZO. But, i suggested that can the CAG promoter may replace the CMV promoter of this vector, because of the CMV promoter may Cause gene-silencing in some special cell lines