ZDHHC5 Double Nickase Plasmid (h): sc-411833-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZDHHC5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZDHHC5 Double Nickase Plasmid (h) and ZDHHC5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZDHHC5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZDHHC5 Double Nickase Plasmid (h) | sc-411833-NIC | 20 µg | $410.00 | |||
ZDHHC5 Double Nickase Plasmid (h2) | sc-411833-NIC-2 | 20 µg | $410.00 |
ZDHHC5 encodes a DHHC family palmitoyltransferase that catalyzes S-acylation of substrate proteins, thereby regulating membrane association, trafficking, and stability of signaling components. Through dynamic palmitoylation cycles, ZDHHC5 influences processes including receptor and ion channel localization, endocytic recycling, and organization of cell–cell junctions and synaptic membranes. These activities connect ZDHHC5 to pathways governing cytoskeletal remodeling and signal transduction at the plasma membrane and endosomal compartments. Dysregulated protein palmitoylation has been linked to oncogenic signaling, neurobiology, and inflammatory phenotypes, making ZDHHC5 a relevant target for studying how lipid modifications tune cellular homeostasis in human cells.
ZDHHC5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZDHHC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZDHHC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZDHHC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZDHHC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.