Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WDR5 Double Nickase Plasmid (h): sc-402425-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WDR5 Double Nickase Plasmid (h) and WDR5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WDR5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WDR5 Antibody (G-9): sc-393080
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR5 Double Nickase Plasmid (h) | sc-402425-NIC | 20 µg | $410.00 |
WDR5 (WD repeat domain 5) encodes a core chromatin-associated scaffold protein that binds histone H3 and coordinates assembly of COMPASS/MLL complexes responsible for H3K4 methylation at active promoters. Through these interactions, WDR5 regulates transcriptional programs controlling cell cycle progression, lineage specification, and maintenance of stem-like states, and it also interfaces with RNA polymerase II transcriptional elongation and enhancer function. WDR5 participates in epigenetic crosstalk with MYC-dependent gene expression and other chromatin regulators that shape global chromatin accessibility. Dysregulation of WDR5-linked chromatin remodeling and H3K4 methylation has been associated with altered developmental gene expression and oncogenic transcriptional circuits in multiple disease contexts.
WDR5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WDR5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WDR5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WDR5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WDR5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.