Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VASP CRISPR/Cas9 KO Plasmid (m): sc-423654
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VASP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VASP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VASP Antibody (A-11): sc-46668
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VASP CRISPR/Cas9 KO Plasmid (m) | sc-423654 | 20 µg | $397.00 |
Overview
Vasp encodes vasodilator-stimulated phosphoprotein (VASP), an Ena/VASP family actin regulatory protein that coordinates actin filament elongation, focal adhesion dynamics, and membrane protrusion during cell migration. VASP integrates signaling downstream of cAMP/cGMP-dependent protein kinases and Rho-family GTPases to couple phosphorylation state with cytoskeletal remodeling and mechanotransduction. In mouse cells, VASP contributes to platelet and endothelial functions, neurite outgrowth, and immune cell motility through regulation of adhesion, barrier properties, and directed movement. Dysregulated VASP-dependent actin dynamics has been implicated in models of vascular dysfunction, thrombosis-related phenotypes, and altered invasive behavior relevant to inflammation and cancer biology.
VASP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vasp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vasp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vasp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VASP protein expression.
This CRISPR knockout system enables efficient generation of Vasp-deficient cell models for investigation of VASP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vasp exon(s) critical for VASP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vasp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VASP CRISPR/Cas9 KO Plasmid (m) and VASP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vasp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VASP HDR Plasmid (m) and VASP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vasp homology arms to support homology-directed repair at defined Vasp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.