Cart (0)

V-ATPase H Antibody (E-10) - Western Blotting - Image 413897 — V-ATPase H Antibody (E-10): sc-271186. Western blot analysis of V-ATPase H expression in untreated K-562 (A), chemically-treated K-562 (B), untreated HCT-116 (C) and chemically-treated HCT-116 (D) whole cell lysates. β-Actin (C4): sc-47778 used as loading control. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.

V-ATPase H Antibody (E-10): sc-271186. Western blot analysis of V-ATPase H expression in untreated K-562 (A), chemically-treated K-562 (B), untreated HCT-116 (C) and chemically-treated HCT-116 (D) whole cell lysates. β-Actin (C4): sc-47778 used as loading control. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.

V-ATPase H Antibody (E-10): sc-271186

5.0(2)

Write a reviewAsk a question

Datasheets

V-ATPase H Antibody (E-10) is a mouse monoclonal IgG_2a κ provided at 200 µg/ml
specific for an epitope mapping between amino acids 10-41 at the N-terminus of V-ATPase H of human origin
recommended for detection of V-ATPase H of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
m-IgG_2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase H Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase H Antibody (E-10) (see ordering information below).

QUICK LINKS

Support Products

Description

Gene Info

Datasheets and Protocols

Research Information

SEE ALSO...

Additional V-ATPase Antibodies including V-ATPase A1, V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2, V-ATPase G1, V-ATPase G2 and V-ATPase H

V-ATPase H Antibody (E-10) is a mouse monoclonal IgG2a antibody that detects V-ATPase H in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). V-ATPase H, also known as the H subunit of the vacuolar-type H+-ATPase, is a crucial regulatory component of this multisubunit enzyme, which is essential for the acidification of eukaryotic intracellular organelles. V-ATPase H is located in the membrane of these organelles, where V-ATPase H plays a vital role in maintaining pH homeostasis and facilitating various cellular processes, such as nutrient uptake and waste removal. The H subunit is composed of two polypeptides derived from the same gene, and proper functioning is critical for the coupling of ATP hydrolysis to proton transport, thereby ensuring efficient energy conversion within the cell. Anti-V-ATPase H antibody (E-10) enables researchers to gain insights into the dynamics of proton transport and the overall functionality of the V-ATPase complex, which is pivotal for cellular health and metabolism.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

V-ATPase H Antibody (E-10) References:

The Tonoplast H+-ATPase of Acer pseudoplatanus Is a Vacuolar-Type ATPase That Operates with a Phosphoenzyme Intermediate. | Magnin, T., et al. 1995. Plant Physiol. 109: 285-292. PMID: 12228595
Plasmalemmal vacuolar-type H+-ATPase in cancer biology. | Sennoune, SR., et al. 2004. Cell Biochem Biophys. 40: 185-206. PMID: 15054222
Optic nerve compression and retinal degeneration in Tcirg1 mutant mice lacking the vacuolar-type H-ATPase a3 subunit. | Kawamura, N., et al. 2010. PLoS One. 5: e12086. PMID: 20711468
Vacuolar-type proton pump ATPases: roles of subunit isoforms in physiology and pathology. | Sun-Wada, GH. and Wada, Y. 2010. Histol Histopathol. 25: 1611-20. PMID: 20886440
Structural bases of physiological functions and roles of the vacuolar H(+)-ATPase. | Ma, B., et al. 2011. Cell Signal. 23: 1244-56. PMID: 21397012
Vacuolar-type H+-ATPase V1A subunit is a molecular partner of Wolfram syndrome 1 (WFS1) protein, which regulates its expression and stability. | Gharanei, S., et al. 2013. Hum Mol Genet. 22: 203-17. PMID: 23035048
Increased production of reactive oxygen species by the vacuolar-type (H(+))-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells. | Yokomakura, A., et al. 2012. J Toxicol Sci. 37: 1045-8. PMID: 23038011
The Vacuolar H+-ATPase B1 Subunit Polymorphism p.E161K Associates with Impaired Urinary Acidification in Recurrent Stone Formers. | Dhayat, NA., et al. 2016. J Am Soc Nephrol. 27: 1544-54. PMID: 26453614
Extra-renal locations of the a4 subunit of H(+)ATPase. | Golder, ZJ. and Karet Frankl, FE. 2016. BMC Cell Biol. 17: 27. PMID: 27368196
Adipocytes control food intake and weight regain via Vacuolar-type H+ ATPase. | Zapata, RC., et al. 2022. Nat Commun. 13: 5092. PMID: 36042358

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	ATP6V1H	Entrez Gene ID: 51606	8q11.23	XM_006716455, XM_011517542, NM_015941, NM_213619, NM_213620	UniProt ID: Q9UI12	608861
Mouse	Atp6v1h	Entrez Gene ID: 108664	1 A1	XM_006495433, XM_006495436, XM_006495437, XM_006495439, XM_011238336, XM_011238337, XM_017321103, NM_001310442, NM_133826	UniProt ID: Q8BVE3	N/A

Ordering Information

Product Name	Catalog #	UNIT	Price	Qty	FAVORITES
V-ATPase H Antibody (E-10)	sc-271186	200 µg/ml	$322.00
V-ATPase H Antibody (E-10): m-IgGκ BP-HRP Bundle	sc-534888	200 µg Ab; 40 µg BP	$361.00
V-ATPase H Antibody (E-10): m-IgG_2a BP-HRP Bundle	sc-546199	200 µg Ab; 10 µg BP	$361.00
V-ATPase H (E-10) Neutralizing Peptide	sc-271186 P	100 µg/0.5 ml	$69.00

Santa Cruz Animal Health is a leading provider of animal health care products. ©2026 Santa Cruz Biotechnology Inc. All Rights Reserved.
Toll-Free: 1-800-457-3801 - Terms & Conditions | Privacy Policy | Legal
“Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.

V-ATPase H Antibody (E-10): sc-271186

V-ATPase H Antibody (E-10) References:

The preferred V-ATPase H antibody is V-ATPase H Antibody (C-8): sc-166227.