Date published: 2025-9-8

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Anticorps UDP-GlcDH (A-9): sc-393438

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Fiches techniques
  • L'Anticorps UDP-GlcDH (A-9) est un monoclonal de souris IgG1 (chaîne légère kappa) fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 459-486 au niveau C-terminus de UDP-GlcDH d'origine human
  • recommandé pour la détection de UDP-GlcDH d'origine mouse, rat et human par WB, IP, IF et ELISA
  • 1 BP-HRP">m-IgG1 BP-HRP est le réactif de détection secondaire préféré pour l'anticorps UDP-GlcDH (A-9) pour les applications WB. Ce réactif est désormais proposé en association avec l'anticorps UDP-GlcDH (A-9)(voir les informations relatives à la commande ci-dessous).

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    UDP-GlcDH (also called UDP-glucose 6-dehydrogenase, UGDH or UDPGDH) is a member of the UDP-glucose/GDP-mannose dehydrogenase family. UDP-GlcDH converts UDP-glucose to UDP-glucuronic acid, which is a crucial component in the biosynthesis of the glycosaminoglycans, hyaluronan, heparan sulfate and chondroitin sulfate. Found as common components of the extracellular matrix, these glycosaminoglycans are significant in signal transduction, cell migration, cancer growth and cancer metastasis. UDP-glucuronic acid (UDP-GlcA) is needed in the liver for the excretion of toxic compounds. UDP-GlcDH is a ubiquitously expressed protein most abundant in the liver. The protein structure of UDP-GlcDH was first analyzed in cow liver and found to be a homohexamer. This structure is well conserved between species and phyla with an overall 97% sequence identity shared between different species of mammals. Research indicates that UDP-GlcDH expression is upregulated by TGFβ and downregulated by hypoxia.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps UDP-GlcDH (A-9)

    sc-393438
    200 µg/ml
    $316.00

    UDP-GlcDH (A-9): m-IgG1 BP-HRP Kit

    sc-542711
    200 µg Ab; 20 µg BP
    $354.00

    UDP-GlcDH (A-9) peptide neutralisant

    sc-393438 P
    100 µg/0.5 ml
    $68.00

    What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

    Posée par: Joy N
    Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
    Répondue par: Tech Support Europe
    Date de publication: 2020-07-09

    What application is the blocking peptide sc-393438 P appropriate for?

    Posée par: Germaine
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Répondue par: Technical Support
    Date de publication: 2017-02-28
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    Rated 5 de 5 de par Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date de publication: 2015-02-23
    Rated 5 de 5 de par Nice Western Blot data of UDPNice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
    Date de publication: 2014-04-23
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    Anticorps UDP-GlcDH (A-9) est évalué 5.0 de 5 de 2.
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