Anticorps UDP-GlcDH (A-9) recommandé pour la détection de UDP-GlcDH d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps UDP-GlcDH (A-9): sc-393438

Anticorps UDP-GlcDH (A-9) est évalué 5.0 de 5 de 2.
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Fiches techniques

    • L'Anticorps UDP-GlcDH (A-9) est un monoclonal de souris IgG1 (chaîne légère kappa) fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 459-486 au niveau C-terminus de UDP-GlcDH d'origine human
    • recommandé pour la détection de UDP-GlcDH d'origine mouse, rat et human par WB, IP, IF et ELISA
    • peptide bloquant, sc-393438 P
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.

What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

Posée par: Joy N
Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
Répondue par: Tech Support Europe
Date de publication: 2020-07-09

What application is the blocking peptide sc-393438 P appropriate for?

Posée par: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-28
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Rated 5 de 5 de par Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date de publication: 2015-02-23
Rated 5 de 5 de par Nice Western Blot data of UDP Nice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
Date de publication: 2014-04-23
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