Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TXNL1 CRISPR/Cas9 KO Plasmid (h): sc-407201
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TXNL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TXNL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TXNL1 Antibody (F-6): sc-515218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TXNL1 CRISPR/Cas9 KO Plasmid (h) | sc-407201 | 20 µg | $397.00 |
Overview
TXNL1 (thioredoxin-like protein 1) is a cytosolic thioredoxin-fold oxidoreductase that supports cellular redox homeostasis by modulating protein thiol–disulfide status and protecting against oxidative stress. Through its reducing activity, TXNL1 can influence redox-sensitive signaling, protein quality control, and pathways linked to metabolism and stress adaptation, including processes that affect cell-cycle progression and survival. Dysregulated redox control and altered TXNL1 expression have been reported in studies of cancer biology and other conditions characterized by oxidative damage, making it a useful target for mechanistic investigation of redox-dependent phenotypes.
TXNL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TXNL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TXNL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TXNL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TXNL1 protein expression.
This CRISPR knockout system enables efficient generation of TXNL1-deficient cell models for investigation of TXNL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TXNL1 exon(s) critical for TXNL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TXNL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TXNL1 CRISPR/Cas9 KO Plasmid (h) and TXNL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TXNL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TXNL1 HDR Plasmid (h) and TXNL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TXNL1 homology arms to support homology-directed repair at defined TXNL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.