Date published: 2025-9-8

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Anticorps TTP (H-12): sc-398904

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Fiches techniques
  • L'Anticorps TTP (H-12) est un monoclonal de souris IgG2b (chaîne légère kappa) fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 287-302 près de C-terminus de TTP d'origine human
  • recommandé pour la détection de TTP d'origine mouse, rat et human par WB, IP, IF et ELISA
  • Voir TTP (A-8): sc-374305 pour d'autres anticorps conjugués TTP, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • 2b BP-HRP">m-IgG2b BP-HRP et m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps TTP (H-12) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps TTP (H-12)(voir les informations relatives à la commande ci-dessous).

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    Tristetraprolin (TTP), also known as Nup475 and TIS11, is a zinc-binding protein encoded by the immediate-early response gene, Zfp-36. Stimulation of quiescent fibroblasts by mitogens, including platelet derived growth factor and fibroblast growth factor, results in the serine phosphorylation of TTP and the rapid redistribution of the protein from the nucleus to the cytoplasm. In vitro studies have demonstrated that TTP is phosphorylated by p42 MAP kinase, indicating that the activity of TTP may be regulated by the MAP kinase pathway in vivo. Knockout mice deficient in TTP develop autoimmunity, inflammatory arthritis and dermatitis. These conditions can be reversed by blocking the activity of the inflammatory mediator, tumor necrosis factor-alpha (TNF-α), suggesting that TTP may function to negatively regulate the expression of TNF-a.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps TTP (H-12)

    sc-398904
    200 µg/ml
    $316.00

    TTP (H-12): m-IgGκ BP-HRP Kit

    sc-536175
    200 µg Ab; 40 µg BP
    $354.00

    TTP (H-12): m-IgG2b BP-HRP Kit

    sc-549522
    200 µg Ab; 10 µg BP
    $354.00

    TTP (H-12) peptide neutralisant

    sc-398904 P
    100 µg/0.5 ml
    $68.00

    Hello, I recently used this Ab for WB with lung homogenates from experimental mice. Even though I saw a clear band, the size of the band was unusually high (above 100kDa). Could you give me an insight about why that could be the case?

    Posée par: STlab
    Thank you for your question. We do not have any reference or data supporting that high of a molecular weight for TTP. We would recommend that you include a positive control in your Western blot testing, such as 3T3-L1, or PC-12 whole cell lysates for comparison. Please contact our Technical Service Department if you would like help troubleshooting the antibody.
    Répondue par: Technical Support
    Date de publication: 2019-01-26

    Has this antibody been shown to detect endogenous levels of TTP in mouse Raw 264.7 cells?

    Posée par: Acarreno13
    Thank you for your question. We have not yet tested this antibody, TTP (H-12): sc-398904, in RAW 264.7 cells. However, we do have endogenous data in other mouse cell lines including RAW 309 Cr.1 and 3T3-L1 whole cell lysates.
    Répondue par: Technical Support
    Date de publication: 2017-12-07

    What application is the blocking peptide sc-398904 P appropriate for?

    Posée par: cjMara
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Répondue par: Technical Support
    Date de publication: 2017-02-24
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    Rated 5 de 5 de par Satisfactory Western blot data of TTP expressionSatisfactory Western blot data of TTP expression in non-transfected and human TTP transfected 293T whole cell lysates. -SCBT QC
    Date de publication: 2013-12-15
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    Anticorps TTP (H-12) est évalué 5.0 de 5 de 1.
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