TOX Antibody (H-2): sc-374137

TOX Antibody (H-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects TOX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TOX antibody (H-2) is available as the non-conjugated form. TOX, or Thymocyte selection-associated high mobility group (HMG) box protein, is a crucial 526 amino acid nuclear protein belonging to the HMG box family of DNA-binding proteins, which plays a significant role in T-cell development regulation. TOX expression is notably upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes, but not in mature thymocytes, highlighting TOX importance in early T-cell maturation stages. TOX (H-2) antibody is essential for CD4 T cell transition, as evidenced by CD4 T cell development failure in TOX-deficient mice, while functional CD8(+) T cells can still develop. This indicates that TOX-dependent transition to the CD4(+)CD8(+) stage is critical for class II major histocompatibility complex-specific T cell development. Additionally, calcineurin activation appears linked to CD8 lineage commitment, as TOX upregulation in double-positive thymocytes depends on calcineurin activity, underscoring TOX pivotal role in T-cell differentiation and immune response.

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TOX Antibody (H-2) References:

1. TOX: an HMG box protein implicated in the regulation of thymocyte selection.  |  Wilkinson, B., et al. 2002. Nat Immunol. 3: 272-80. PMID: 11850626
2. TOX provides a link between calcineurin activation and CD8 lineage commitment.  |  Aliahmad, P., et al. 2004. J Exp Med. 199: 1089-99. PMID: 15078895
3. Receptor signals and nuclear events in CD4 and CD8 T cell lineage commitment.  |  Laky, K. and Fowlkes, BJ. 2005. Curr Opin Immunol. 17: 116-21. PMID: 15766669
4. Commitment issues: linking positive selection signals and lineage diversification in the thymus.  |  Aliahmad, P. and Kaye, J. 2006. Immunol Rev. 209: 253-73. PMID: 16448547
5. TCR and Notch signaling in CD4 and CD8 T-cell development.  |  Laky, K., et al. 2006. Immunol Rev. 209: 274-83. PMID: 16448548
6. Development of all CD4 T lineages requires nuclear factor TOX.  |  Aliahmad, P. and Kaye, J. 2008. J Exp Med. 205: 245-56. PMID: 18195075
7. Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats.  |  Mitnacht, R., et al. 1998. J Immunol. 160: 700-7. PMID: 9551905
8. Positive and negative thymocyte selection.  |  Saito, T. and Watanabe, N. 1998. Crit Rev Immunol. 18: 359-70. PMID: 9704194

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TOX Antibody (H-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TOX Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TOX Antibody (H-2) Specifications

Alternate Product Name: TOX

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 31-63

Epitope Target: TOX

Epitope Species: human

Product Reactivity/Detection: TOX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TOX Antibody (H-2): P: Blocking Peptide