Anticorps Tom40 (D-2) recommandé pour la détection de Tom40 d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps Tom40 (D-2): sc-365467

Anticorps Tom40 (D-2) est évalué 4.5 de 5 de 12.
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Fiches techniques

    • Anti-L'anticorps Tom40 D-2 est un monoclonal IgG1 κ L'Anticorps Tom40, cité dans 26 publications, fourni en 200 µg/ml
    • dirigé contre les acides aminés 62-361 de Tom40 d'origine human
    • Anti-L'Anticorps Tom40 (D-2) est recommandé pour la détection de Tom40 d'origine mouse, rat et human par WB, IP, IF et ELISA
    • Anti-L'Anticorps Tom40 (D-2) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Tom40 (D-2): sc-365467.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Tom40 Antibody (D-2) for WB applications. This reagent is now offered in a bundle with Tom40 Antibody (D-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

since this Abs is available in agrose form for coIP experiment. Then I guess this antibody (non-conjugated) is OK to use for coIP together with protein G beads, right?

Posée par: Zach
The primary antibody (both unconjugated and agarose-conjugated forms) is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Répondue par: Technical Support
Date de publication: 2019-06-16

What is the recommended fixation method for immunofluorescence staining with Tom40 (D-2): sc-365467 monoclonal antibody?

Posée par: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 4 de 5 de par Monoclonal de souris anti TOM 40 cet anticorps fonctionne très bien sur des lysats de cellules et de globules rouges
Date de publication: 2019-09-19
Rated 4 de 5 de par no crossreactivity in C. elegans detected we wanted to test whether this antibody against human TOM40 might crossreact with the ortholog in the nematode C. elegans. we detected a band at expected molecular weight, however, the antibody also detected other proteins unspecifically.
Date de publication: 2018-04-27
Rated 5 de 5 de par working working in HEK cells in concentration 1:100 in HEK293 cells
Date de publication: 2018-02-20
Rated 4 de 5 de par Good for WB I used this Ab for mitochondria isolation. Just get weak bend in my situation but suitable for WB in 1:500 dilution.
Date de publication: 2017-09-27
Rated 4 de 5 de par Good signal in WB Expected band in mouse macrophage cell line, 1/150.
Date de publication: 2017-08-24
Rated 5 de 5 de par Nice band This antibody was recently re-tested in Hela cell lysates . A nice signal was observed and no background.I highly recommend using this antibody with the recommend protocol on santa website.
Date de publication: 2017-07-23
Rated 5 de 5 de par Strong Detection of Tom40 sc-365467 shows great detection of Tom40 is Western Blot with human cell lysates.
Date de publication: 2017-01-19
Rated 4 de 5 de par Further verication needed I tried the antibody. I got a very nice band at the right size in 293T cells. However, when I transfected 293T cells with empty vector or Tom40-HA expression vector (sequencing confirmed that the construct was right), I did not see a significant increase in Tom40 levels in 293T cells overexpressing Tom40-HA compared to vector control cells by WB using this antibody, but using anti-HA antibody, a very strong band with the right size was shown up in the lane of Tom40-HA overexpressing cell lysate.
Date de publication: 2016-12-23
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