Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TDP1 CRISPR/Cas9 KO Plasmid (h): sc-405057
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TDP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TDP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TDP1 Antibody (C-3): sc-365674
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TDP1 CRISPR/Cas9 KO Plasmid (h) | sc-405057 | 20 µg | $397.00 |
Overview
Tyrosyl-DNA phosphodiesterase 1 (TDP1) is a nuclear DNA repair enzyme that hydrolyzes covalent 3′-phosphotyrosyl linkages formed when topoisomerase I becomes trapped on DNA, as well as other blocked 3′ DNA termini. Through coordination with single-strand break repair and base excision repair pathways, including interactions with PARP1, XRCC1, and DNA ligase III, TDP1 helps restore ligatable ends and preserve genome stability during transcription and replication stress. Loss or impairment of TDP1 disrupts resolution of Top1-DNA adducts, increasing accumulation of DNA strand breaks and replication-associated lesions. TDP1 dysfunction is linked to neurodegenerative phenotypes such as spinocerebellar ataxia with axonal neuropathy and is broadly relevant to studies of DNA damage tolerance and cellular responses to genotoxic stress.
TDP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TDP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TDP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TDP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TDP1 protein expression.
This CRISPR knockout system enables efficient generation of TDP1-deficient cell models for investigation of TDP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TDP1 exon(s) critical for TDP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TDP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TDP1 CRISPR/Cas9 KO Plasmid (h) and TDP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TDP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TDP1 HDR Plasmid (h) and TDP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TDP1 homology arms to support homology-directed repair at defined TDP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.