striatin Double Nickase Plasmid (h): sc-406215-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- striatin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- striatin Double Nickase Plasmid (h) and striatin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STRN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: striatin Antibody (6): sc-136084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
striatin Double Nickase Plasmid (h) | sc-406215-NIC | 20 µg | $410.00 | |||
striatin Double Nickase Plasmid (h2) | sc-406215-NIC-2 | 20 µg | $410.00 |
Human STRN encodes striatin, a calmodulin-binding scaffolding protein within the STRIPAK (striatin-interacting phosphatase and kinase) complex that coordinates PP2A-associated signaling, kinase regulation, and assembly of multiprotein complexes at cellular membranes and junctional sites. Through these interactions, striatin contributes to integration of calcium-dependent cues with pathways controlling cytoskeletal dynamics, cell polarity, vesicular trafficking, and proliferation-associated signaling networks. Altered STRN/STRIPAK function has been linked to dysregulated signal transduction and junctional organization in contexts relevant to oncogenic pathway remodeling and tissue-specific homeostasis. These properties make STRN a useful target for dissecting scaffold-mediated pathway crosstalk and phosphatase–kinase balance in human cells.
striatin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STRN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STRN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STRN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STRN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.