SRPK1 CRISPR/Cas9 KO Plasmid (m): sc-423158
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SRPK1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SRPK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRPK1 CRISPR/Cas9 KO Plasmid (m) | sc-423158 | 20 µg | $397.00 |
Overview
Mouse Srpk1 encodes serine/arginine-rich protein-specific kinase 1 (SRPK1), a key regulator of pre-mRNA splicing through phosphorylation of SR proteins such as SRSF factors, influencing their nuclear localization and spliceosome assembly. By coupling signaling cues to alternative splicing decisions, SRPK1 impacts gene expression programs that govern proliferation, stress responses, and differentiation. SRPK1 activity intersects with RNA processing networks linked to cell-cycle control and post-transcriptional regulation, making it relevant to studies of dysregulated splicing observed in cancer, neurodegeneration, and inflammatory settings. In mouse systems, SRPK1 perturbation is commonly used to dissect how splicing kinase signaling shapes tissue-specific transcript isoforms and downstream phenotypes.
SRPK1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Srpk1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Srpk1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Srpk1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SRPK1 protein expression.
This CRISPR knockout system enables efficient generation of Srpk1-deficient cell models for investigation of SRPK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Srpk1 exon(s) critical for SRPK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Srpk1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SRPK1 CRISPR/Cas9 KO Plasmid (m) and SRPK1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Srpk1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SRPK1 HDR Plasmid (m) and SRPK1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Srpk1 homology arms to support homology-directed repair at defined Srpk1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.