Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Squalene epoxidase Double Nickase Plasmid (h): sc-402870-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Squalene epoxidase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Squalene epoxidase Double Nickase Plasmid (h) and Squalene epoxidase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SQLE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Squalene epoxidase Antibody (H-6): sc-271651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Squalene epoxidase Double Nickase Plasmid (h) | sc-402870-NIC | 20 µg | $410.00 | |||
Squalene epoxidase Double Nickase Plasmid (h2) | sc-402870-NIC-2 | 20 µg | $410.00 |
SQLE encodes squalene epoxidase, a flavin-dependent monooxygenase that catalyzes the epoxidation of squalene to 2,3-oxidosqualene, an early rate-influencing step in cholesterol and sterol biosynthesis. As an endoplasmic reticulum–associated enzyme, it helps regulate sterol homeostasis and integrates with mevalonate pathway flux, lipid droplet dynamics, and feedback control of membrane composition. Perturbation of SQLE activity can reshape cellular lipid profiles, impacting signaling platforms and metabolic adaptation under stress. Altered sterol biosynthetic regulation involving SQLE has been associated with dyslipidemia-related phenotypes and metabolic reprogramming observed in multiple disease contexts, supporting its use as a mechanistic node in lipid metabolism research.
Squalene epoxidase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SQLE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SQLE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SQLE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SQLE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.