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Anticorps SH2-B α/β/γ/δ (46): sc-136065

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Fiches techniques
  • L'anticorps SH2-B alpha / beta / gamma / delta 46 est un monoclonal IgG1 de souris, cité dans 6 publications, fourni en 50 µg/0.5 ml
  • dirigé contre les acides aminés 403-513 de SH2-Bβ d'origine rat
  • recommandé pour la détection de SH2-B α, β, γ and δ d'origine mouse, rat et human par WB, IP et IF
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire SH2-B α/β/γ/δ (46). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

SH2-B, also known as SH2B1 or PSM, is a 756 amino acid protein that is a component of the signaling network and is involved in the regulation of cell shape and movement. SH2-B is related to the APS (adapter molecule containing PH and SH2 domains) family of adapter proteins, which characteristically contain a pleckstrin homology (PH) domain, an SH2 domain and a tyrosine phosphorylation site. SH2-B is alternatively spliced to generate three distinct isoforms, SH2-B α, β, and γ, that share an identical N-terminal sequence, including the PH domain, the SH2 domain, and multiple proline-rich motifs. Containing a PH domain and a SH2 domain, SH2-B shuttles between the nucleus and the cytoplasm. SH2-B is widely expressed with highest expression in skeletal muscle and ovary. SH2-B is phosphorylated on tyrosine residues in response to receptor kinase stimulation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps SH2-B α/β/γ/δ (46)

sc-136065
50 µg/0.5 ml
$316.00

I am using SH2-B α/β/γ/δ (46): sc-136065 for immunofluorescence, what dilution of this antibody should I use?

Posée par: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 5 de 5 de par Immunoblots endogenous SH2B1 in PC12 cellsAnti-SH2B1 (46) against aa 403-513 of rat SH2B1 (sc. 136065) PC12 cells stably expressing GFP tag (only endogenous SH2B1 present). Treated ± NGF (100 ng/ml), cells lysed in RIPA buffer (1% Triton X100). Load 40 ul on minigel. Immunoblot: block with 3% BSA in TBS-1% tween, blot with anti-SH2B1 (46) (1/250 dilution). 2o antibody: mIGGkBP-HRP (sc) (1/1000). Nice SH2B1 signal detected with NGF-dependent shift in migration. Band at 90 kDa in absence of NGF. Band at 100 kDa in sample treated with NGF. 293T cell transfected with the four isoforms of human GFP-SH2B1. Cells lysed in RIPA buffer (1% Triton X100). Load 20 ul on minigel. Immunoblot: block with 3% BSA in TBS-1% tween, blot with anti-SH2B1 (46) (1/1000 dilution). 2o antibody: anti-mouse IRdye680 (1/20,000). The four isoforms of SH2B1 were detected by the SH2B1 (46) antibody. For most antibodies prepared against SH2B1, the background is diminished when a 2o antibody conjugated to HRP is used (when compared to 2o antibodies conjugated to IRdyes.)
Date de publication: 2018-09-12
Rated 5 de 5 de par Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear and cytoplasmic staining in HeLa cells. -SCBT QC
Date de publication: 2015-03-23
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Anticorps SH2-B α/β/γ/δ (46) est évalué 5.0 de 5 de 3.
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