Date published: 2025-9-7

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Anticorps RPA 32 kDa subunit (B-4): sc-271578

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Fiches techniques
  • L'Anticorps RPA 32 kDa subunit (B-4) est un monoclonal de souris IgG2b κ, cité dans 3 publications, fourni en 200 µg/ml
  • soulevée à l'encontre des acides aminés 155-254 correspondant à une région interne de RPA 32 kDa subunit d'origine human.
  • recommandé pour la détection de RPA 32 kDa subunit d'origine human par WB, IP, IF et ELISA
  • Réactifs TransCruz pour une application en ChIP (sc-271578 X, 200 µg/0.1 ml)
  • Voir RPA 32 kDa subunit (9H8): sc-56770 pour d'autres anticorps conjugués RPA 32 kDa subunit, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • 2b BP-HRP">m-IgG2b BP-HRP est le réactif de détection secondaire préféré pour l'anticorps RPA 32 kDa subunit (B-4) pour les applications WB. Ce réactif est désormais proposé en association avec l'anticorps RPA 32 kDa subunit (B-4)(voir les informations relatives à la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of a ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps RPA 32 kDa subunit (B-4)

sc-271578
200 µg/ml
$316.00

RPA 32 kDa subunit (B-4): m-IgG2b BP-HRP Kit

sc-548542
200 µg Ab; 10 µg BP
$354.00

Anticorps RPA 32 kDa subunit (B-4) X

sc-271578 X
200 µg/0.1 ml
$316.00

What is the recommended fixation method for immunofluorescence staining with RPA 32 kDa subunit (B-4): sc-271578 monoclonal antibody?

Posée par: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date de publication: 2015-02-20
Rated 5 de 5 de par Good Western Blot data of RPA 32 kDa subunitGood Western Blot data of RPA 32 kDa subunit expression in HeLa, H69AR and T-47D whole cell lysates. -SCBT QC
Date de publication: 2014-09-29
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Anticorps RPA 32 kDa subunit (B-4) est évalué 5.0 de 5 de 2.
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