Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ribosomal Protein S3 CRISPR/Cas9 KO Plasmid (h): sc-402327
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein S3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ribosomal Protein S3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ribosomal Protein S3 Antibody (C-7): sc-376008
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein S3 CRISPR/Cas9 KO Plasmid (h) | sc-402327 | 20 µg | $397.00 |
Overview
RPS3 encodes ribosomal protein S3, a core component of the 40S small ribosomal subunit that supports translation initiation, decoding, and overall ribosome biogenesis. Beyond its structural role in protein synthesis, RPS3 has been linked to cellular stress responses and can influence signaling networks connected to transcriptional regulation, apoptosis, and DNA damage responses. Because ribosome function constrains proliferation and proteome homeostasis, altered RPS3 expression or ribosomal imbalance is frequently examined in contexts of oncogenic growth, inflammatory signaling, and neurodegenerative stress. RPS3 perturbation is therefore useful for dissecting how translational control interfaces with genome maintenance and proteostasis pathways.
Ribosomal Protein S3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RPS3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RPS3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RPS3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ribosomal Protein S3 protein expression.
This CRISPR knockout system enables efficient generation of RPS3-deficient cell models for investigation of Ribosomal Protein S3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RPS3 exon(s) critical for Ribosomal Protein S3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RPS3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ribosomal Protein S3 CRISPR/Cas9 KO Plasmid (h) and Ribosomal Protein S3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RPS3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ribosomal Protein S3 HDR Plasmid (h) and Ribosomal Protein S3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RPS3 homology arms to support homology-directed repair at defined RPS3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.