Rag B Antibody (2B8): sc-293349

Rag B Antibody (2B8) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Rag B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Rag B antibody (2B8) is available as a non-conjugated antibody, providing researchers with a reliable tool for studying this important protein. Rag B, also known as RRAGB, is a 374 amino acid cytoplasmic protein that plays a crucial role in cellular signaling and metabolism. Rag B (2B8) antibody belongs to the GTR/RAG GTP-binding protein family and is essential for the activation of the mechanistic target of rapamycin complex 1 (mTORC1) in response to amino acids. Rag B (2B8) monoclonal antibody′s function is vital for regulating cell growth and proliferation, as mTORC1 is a central controller of cellular metabolism, growth, and autophagy. Rag B exists in both short and long isoforms due to alternative splicing, with the short isoform being GTP-bound and active. This GTP-binding activity is critical for the relocalization of mTORC1 to the lysosomal surface, where mTORC1 can be activated by RHEB, another GTPase. Understanding the dynamics of Rag B and its interactions is essential for elucidating the mechanisms of nutrient sensing and signaling pathways that influence cellular homeostasis and disease states.

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Rag B Antibody (2B8) References:

1. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B.  |  Sekiguchi, T., et al. 2001. J Biol Chem. 276: 7246-57. PMID: 11073942
2. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1.  |  Sancak, Y., et al. 2008. Science. 320: 1496-501. PMID: 18497260
3. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.  |  Sancak, Y., et al. 2010. Cell. 141: 290-303. PMID: 20381137
4. Cloning of a novel family of mammalian GTP-binding proteins (RagA, RagBs, RagB1) with remote similarity to the Ras-related GTPases.  |  Schürmann, A., et al. 1995. J Biol Chem. 270: 28982-8. PMID: 7499430
5. Mapping of Ras-related GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis.  |  Huber, LA., et al. 1994. Proc Natl Acad Sci U S A. 91: 7874-8. PMID: 8058727
6. RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway.  |  Hirose, E., et al. 1998. J Cell Sci. 111 (Pt 1): 11-21. PMID: 9394008

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rag B Antibody (2B8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Rag B Antibody (2B8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rag B Antibody (2B8) Specifications

Alternate Product Name: Rag B

Product Clone Name: 2B8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-346

Epitope Target: Rag B

Epitope Species: human

Product Reactivity/Detection: Rag B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Rag B Antibody (2B8): N/A