Rab 5 Antibody (D-11): sc-46692
- Rab 5 Antibody (D-11) is a mouse monoclonal IgG2b κ Rab 5 antibody, cited in 112 publications, provided at 200 µg/ml
- raised against amino acids 1-215 representing full length Rab 5AA of human origin
- Rab 5 Antibody (D-11) is recommended for detection of Rab 5A, 5B and 5C; partially cross reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- Anti-Rab 5 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 5 Antibody (D-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Rab 5 Antibody (D-11) (see ordering information below).
Rab 5 Antibody (D-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Rab 5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Rab 5 (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 5 protein plays a crucial role in regulating endocytosis and protein transport within cells, particularly in moving carrier vesicles from endoplasmic reticulum to Golgi apparatus and ultimately to secretory vesicles. This process maintains cellular homeostasis and facilitates communication between different cellular compartments. Rab 5 interacts with various effector proteins essential for vesicle docking and fusion, ensuring proteins are delivered to correct destinations. Proper functioning of Rab 5 is critical, as disruptions in activity can lead to various diseases, including neurodegenerative disorders and cancer, highlighting studying importance in both basic and clinical research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rab 5 Antibody (D-11) References:
- GTP-binding proteins in intracellular transport. | Pfeffer, SR. 1992. Trends Cell Biol. 2: 41-6. PMID: 14731525
- The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach. | Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
- Cloning of a Rab3 isotype predominantly expressed in adipocytes. | Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
- Rab 5 is required for the cellular entry of dengue and West Nile viruses. | Krishnan, MN., et al. 2007. J Virol. 81: 4881-5. PMID: 17301152
- RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion. | Smurova, K. and Podbilewicz, B. 2016. Cell Rep. 14: 1517-1527. PMID: 26854231
- LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling. | Liu, H., et al. 2018. J Cell Biol. 217: 299-314. PMID: 29079669
- Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag. | Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
- Coatomers and SNAREs in promoting membrane traffic. | Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
- Friends and family: the role of the Rab GTPases in vesicular traffic. | Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
- The role of GTP-binding proteins in transport along the exocytic pathway. | Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
- Association of the low molecular weight GTP-binding protein rap2B with the cytoskeleton during platelet aggregation. | Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
- Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins. | Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 5 Antibody (D-11) | sc-46692 | 200 µg/ml | $322.00 | |||
Rab 5 Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-528501 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rab 5 Antibody (D-11): m-IgGκ BP-HRP Bundle | sc-520893 | 200 µg Ab, 40 µg BP | $361.00 | |||
Rab 5 Antibody (D-11): m-IgG2b BP-HRP Bundle | sc-550046 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rab 5 Antibody (D-11) AC | sc-46692 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rab 5 Antibody (D-11) HRP | sc-46692 HRP | 200 µg/ml | $322.00 | |||
Rab 5 Antibody (D-11) FITC | sc-46692 FITC | 200 µg/ml | $336.00 | |||
Rab 5 Antibody (D-11) PE | sc-46692 PE | 200 µg/ml | $349.00 | |||
Rab 5 Antibody (D-11) Alexa Fluor® 488 | sc-46692 AF488 | 200 µg/ml | $364.00 | |||
Rab 5 Antibody (D-11) Alexa Fluor® 546 | sc-46692 AF546 | 200 µg/ml | $364.00 | |||
Rab 5 Antibody (D-11) Alexa Fluor® 594 | sc-46692 AF594 | 200 µg/ml | $364.00 | |||
Rab 5 Antibody (D-11) Alexa Fluor® 647 | sc-46692 AF647 | 200 µg/ml | $364.00 | |||
Rab 5 Antibody (D-11) Alexa Fluor® 680 | sc-46692 AF680 | 200 µg/ml | $364.00 | |||
Rab 5 Antibody (D-11) Alexa Fluor® 790 | sc-46692 AF790 | 200 µg/ml | $364.00 |