PPARα Antibody (H-2): sc-398394

PPARα Antibody (H-2) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Peroxisome Proliferator-Activated Receptor Alpha (PPARα), also known as NR1C1, PPARA, or peroxisome proliferator activated receptor alpha. PPARα monoclonal antibody (H-2) is raised against amino acids 1-98 of the human PPARα protein, ensuring effective recognition in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) across multiple species such as mouse, rat, and human. PPARα is a nuclear hormone receptor characterized by its modular structure, which includes a DNA-binding domain, a ligand-binding domain, and an N-terminal activation domain. This structural configuration is essential for PPARα′s function in regulating gene expression, as PPARα forms heterodimers with retinoid X receptors (RXRs) and binds to specific PPAR-response elements (PPREs) within the DNA. Located predominantly in the nucleus, especially abundant in primary hepatocytes, PPARα plays a crucial role in controlling the transcription of genes involved in fatty acid metabolism, lipid homeostasis, and energy balance. The precise structure of PPARα facilitates interaction with various ligands, such as fibrates and fatty acids, which activate transcriptional activity, and enables interaction with other nuclear receptors like LXRα and thyroid receptors that can modulate DNA binding. Understanding the structural dynamics of PPARα is vital for elucidating its role in metabolic processes and involvement in conditions such as metabolic disorders, cardiovascular diseases, and liver function. Anti-PPARα antibody (H-2) is an invaluable tool for researchers studying the intricate mechanisms of PPARα-mediated pathways, providing reliable detection and analysis that support advancements in molecular biology and therapeutic development.

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PPARα Antibody (H-2) References:

1. Integrated physiology and systems biology of PPARα.  |  Kersten, S. 2014. Mol Metab. 3: 354-71. PMID: 24944896
2. The potential of natural products for targeting PPARα.  |  Rigano, D., et al. 2017. Acta Pharm Sin B. 7: 427-438. PMID: 28752027
3. Dietary excess regulates absorption and surface of gut epithelium through intestinal PPARα.  |  Stojanović, O., et al. 2021. Nat Commun. 12: 7031. PMID: 34857752
4. Therapeutic roles of PPARα activation in ocular ischemic diseases.  |  Lee, D., et al. 2023. Histol Histopathol. 38: 391-401. PMID: 36305579
5. Selective activation of PPARα maintains thermogenic capacity of beige adipocytes.  |  Egusa, G., et al. 2023. iScience. 26: 107143. PMID: 37456852
6. Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-alpha, -beta, and -gamma in the adult rat.  |  Braissant, O., et al. 1996. Endocrinology. 137: 354-66. PMID: 8536636
7. Expression of the peroxisome proliferator-activated receptor alpha gene is stimulated by stress and follows a diurnal rhythm.  |  Lemberger, T., et al. 1996. J Biol Chem. 271: 1764-9. PMID: 8576180
8. Differential activation of adipogenesis by multiple PPAR isoforms.  |  Brun, RP., et al. 1996. Genes Dev. 10: 974-84. PMID: 8608944
9. Regulation of peroxisome proliferator-activated receptor-alpha mRNA in rat liver.  |  Sterchele, PF., et al. 1996. Arch Biochem Biophys. 326: 281-9. PMID: 8611035
10. The orphan nuclear hormone receptor LXR alpha interacts with the peroxisome proliferator-activated receptor and inhibits peroxisome proliferator signaling.  |  Miyata, KS., et al. 1996. J Biol Chem. 271: 9189-92. PMID: 8621574
11. Crosstalk between the thyroid hormone and peroxisome proliferator-activated receptors in regulating peroxisome proliferator-responsive genes.  |  Hunter, J., et al. 1996. Mol Cell Endocrinol. 116: 213-21. PMID: 8647322
12. Expression of the peroxisome proliferator-activated receptor (PPAR) in the mouse colonic mucosa.  |  Mansén, A., et al. 1996. Biochem Biophys Res Commun. 222: 844-51. PMID: 8651933

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PPARα Antibody (H-2) has 174 citations in a variety of scientific publications.

Primary antibodies like Anti-PPARα Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PPARα Antibody (H-2) Specifications

Alternate Product Name: PPAR alpha

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-98

Epitope Target: PPARα

Epitope Species: human

Product Reactivity/Detection: PPARα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 174 citations in various scientific publications

Available Conjugates/Forms for PPARα Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent