POPSO (CAS 68189-43-5)

POPSO (Piperazine-N,N′-bis(2-hydroxypropanesulfonic acid)) is a zwitterionic biological buffer commonly used in biochemical and biophysical research to maintain stable pH conditions across a range of biochemical processes. With a useful pH range from 6.8 to 8.0, POPSO is particularly favored in environments where maintaining a precise, slightly alkaline pH is crucial. The buffer′s mechanism relies on its zwitterionic nature, which allows it to resist changes in pH by reacting to both acids and bases. This makes it an ideal choice for applications such as enzyme kinetics studies, protein purification, and other procedures that require stringent control of environmental conditions to ensure the stability and activity of biological macromolecules. POPSO is also used in electrophoresis and other separation techniques where non-interfering buffering capacity is necessary. The chemical structure of POPSO, incorporating piperazine as a backbone linked with hydroxypropanesulfonic acid groups, ensures minimal interaction with biological molecules, thereby reducing the risk of altering the natural behavior of these molecules during experiments. Its use in research is critical for experiments that demand a tightly controlled pH without the interference from the buffer itself, supporting accurate and reproducible results in biochemical assays and molecular biology.