PKR Antibody (B-10): sc-6282

PKR Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects PKR of mouse, rat, and human origin by WB, IP, IF, and IHCP. PKR Antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. Protein kinase R (PKR), also known as EIF2AK2, plays a crucial role in the cellular response to viral infections by acting as an interferon-inducible, RNA-dependent protein serine/threonine kinase. PKR′s primary function is to phosphorylate the α subunit of the protein synthesis initiation factor eIF-2, specifically at serine-51, which leads to the inhibition of translation and effectively halts viral replication. This mechanism is vital for the host′s defense against viral pathogens, as PKR prevents the production of viral proteins. PKR is characterized by distinct structural domains, with the serine/threonine kinase catalytic domains located in the carboxy-terminal half and the RNA-binding domains situated in the amino-terminal region. Additionally, PKR′s activity is tightly regulated through various mechanisms, including transcriptional regulation in response to interferon, an autoregulatory mechanism that controls PKR expression at the translational level, and post-translational regulation via RNA-mediated autophosphorylation. This multifaceted regulation underscores PKR′s importance in maintaining cellular homeostasis and responding to stress signals.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PKR Antibody (B-10) References:

1. Mechanism of interferon action: cDNA structure, expression, and regulation of the interferon-induced, RNA-dependent P1/eIF-2 alpha protein kinase from human cells.  |  Thomis, DC., et al. 1992. Virology. 188: 33-46. PMID: 1373553
2. Mechanism of interferon action: identification of a RNA binding domain within the N-terminal region of the human RNA-dependent P1/eIF-2 alpha protein kinase.  |  McCormack, SJ., et al. 1992. Virology. 188: 47-56. PMID: 1373554
3. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon.  |  Meurs, E., et al. 1990. Cell. 62: 379-90. PMID: 1695551
4. TIK, a novel serine/threonine kinase, is recognized by antibodies directed against phosphotyrosine.  |  Icely, PL., et al. 1991. J Biol Chem. 266: 16073-7. PMID: 1714905
5. Protein phosphorylation controls translation rates.  |  Hershey, JW. 1989. J Biol Chem. 264: 20823-6. PMID: 2687263
6. RNA circles with minimized immunogenicity as potent PKR inhibitors.  |  Liu, CX., et al. 2022. Mol Cell. 82: 420-434.e6. PMID: 34951963
7. Viral evasion of PKR restriction by reprogramming cellular stress granules.  |  Gao, P., et al. 2022. Proc Natl Acad Sci U S A. 119: e2201169119. PMID: 35858300
8. The mouse antiphosphotyrosine immunoreactive kinase, TIK, is indistinguishable from the double-stranded RNA-dependent, interferon-induced protein kinase, PKR.  |  Baier, LJ., et al. 1993. Nucleic Acids Res. 21: 4830-5. PMID: 7694235
9. Mechanism of interferon action: structure of the mouse PKR gene encoding the interferon-inducible RNA-dependent protein kinase.  |  Tanaka, H. and Samuel, CE. 1994. Proc Natl Acad Sci U S A. 91: 7995-9. PMID: 7914700
10. The eIF-2 alpha protein kinases, regulators of translation in eukaryotes from yeasts to humans.  |  Samuel, CE. 1993. J Biol Chem. 268: 7603-6. PMID: 8096514

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKR Antibody (B-10) has 172 citations in a variety of scientific publications.

Primary antibodies like Anti-PKR Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKR Antibody (B-10) Specifications

Alternate Product Name: PKR

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-515

Epitope Target: PKR

Epitope Species: mouse

Product Reactivity/Detection: PKR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 172 citations in various scientific publications

Available Conjugates/Forms for PKR Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin