Anticorps Pin1 (8C10) recommandé pour la détection de Pin1 d'origine mouse, rat, human et canine par WB et IP

Anticorps Pin1 (8C10): sc-81533

Anticorps Pin1 (8C10) est évalué 5.0 de 5 de 3.
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Fiches techniques

    • L'anticorps Pin1 8C10 est un monoclonal IgG1 de souris, cité dans 2 publications, fourni en 50 µg/0.5 ml
    • dirigé contre la Pin1 recombinante d'origine human
    • recommandé pour la détection de Pin1 d'origine mouse, rat, human et canine par WB et IP
    • Voir Pin1 (G-8): sc-46660 pour d'autres anticorps conjugués Pin1, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Pin1 Antibody (8C10). This work is in progress.
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I am using Pin1 (8C10): sc-81533 antibody for immunoprecipitation, how much antibody should I use?

Posée par: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Répondue par: Technical Support
Date de publication: 2017-03-24

Hi, I wonder what the protein sequence of immunogen is?

Posée par: Yaooo
Thank you for your question. Pin1 Antibody (8C10): sc-81533 is a mouse monoclonal IgG1 raised against recombinant Pin1 of human origin. Unfortunately, the exact recombinant protein sequence used as an immunogen is not known.
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Robust signal seen in WB in Jurkat nuclear extract Robust signal seen in WB in Jurkat nuclear extract. -SCBT QC
Date de publication: 2015-05-23
Rated 5 de 5 de par Strong band observed in WB in HeLa Strong band observed in WB in HeLa, K-562, Jurkat, NIH/3T3, PC-3 and Hep G2 nuclear extracts. -SCBT QC
Date de publication: 2015-02-25
Rated 5 de 5 de par Antibody worked well for WB in serum starved A Antibody worked well for WB in serum starved A-431, A549, SKOV3, OVCAR-5, HaCaT, PC-3, HeLa and Hep G2 whole cell lysates. -SCBT QC
Date de publication: 2014-04-20
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