Date published: 2025-10-27
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western blotting image from recommended PGC1a (D-5) monoclonal antibody replacement. Image 390096
Cet anticorps polyclonal n'est plus commercialisé. Voir l'anticorps monoclonal PGC1a (D-5) que nous recommandons (données d'échantillonnage indiquées).
western blotting image from recommended PGC1a (D-5) monoclonal antibody replacement. Image 390096
Cet anticorps polyclonal n'est plus commercialisé. Voir l'anticorps monoclonal PGC1a (D-5) que nous recommandons (données d'échantillonnage indiquées).
Cet anticorps polyclonal n'est plus commercialisé. Voir l'anticorps monoclonal PGC1a (D-5) que nous recommandons (données d'échantillonnage indiquées).

Anticorps PGC-1α (H-300): sc-13067

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Écrire une critiquePoser une question

  • L'anticorps PGC-1 alpha H-300 est un polyclonal IgG de lapin fourni en 200 µg/ml
  • épitope correspondant aux acides aminés 1-300 situés au niveau N-terminus de PGC-1 d'origine human
  • Anticorps polyclonal arrêté

VOIR ÉGALEMENT...

Anticorps supplémentaires PGC incluant PGC-1 α et PGC-1 β

Informations pour la commande

PGC1a (D-5): sc-518025 [ Monoclonal recommandé en remplacement de PGC-1α (H-300) ]

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps PGC1a (D-5)

sc-518025
200 µg/ml
$316.00

PGC1a (D-5): m-IgG Fc BP-HRP Kit

sc-531597
200 µg Ab; 10 µg BP
$354.00

PGC1a (D-5): m-IgGκ BP-HRP Kit

sc-525331
200 µg Ab, 40 µg BP
$354.00

PGC1a (D-5): m-IgG2a BP-HRP Kit

sc-547825
200 µg Ab; 10 µg BP
$354.00

Anticorps PGC1a (D-5) AC

sc-518025 AC
500 µg/ml, 25% agarose
$416.00

Anticorps PGC1a (D-5) HRP

sc-518025 HRP
200 µg/ml
$316.00

Anticorps PGC1a (D-5) FITC

sc-518025 FITC
200 µg/ml
$330.00

Anticorps PGC1a (D-5) PE

sc-518025 PE
200 µg/ml
$343.00

Anticorps PGC1a (D-5) Alexa Fluor® 488

sc-518025 AF488
200 µg/ml
$357.00

Anticorps PGC1a (D-5) Alexa Fluor® 546

sc-518025 AF546
200 µg/ml
$357.00

Anticorps PGC1a (D-5) Alexa Fluor® 594

sc-518025 AF594
200 µg/ml
$357.00

Anticorps PGC1a (D-5) Alexa Fluor® 647

sc-518025 AF647
200 µg/ml
$357.00

Anticorps PGC1a (D-5) Alexa Fluor® 680

sc-518025 AF680
200 µg/ml
$357.00

Anticorps PGC1a (D-5) Alexa Fluor® 790

sc-518025 AF790
200 µg/ml
$357.00

If the product is specified for mice, can it work on rat tissue¿

Posée par: XimenaYri
Thank you for your question. PGC-1α(H-300) was recommended for detection of PGC-1α of mouse, rat and human origin. This antibody has been discontinued. We recommend you use one of our monoclonal alternatives, such as PGC-1α Anticuerpo (D-5): sc-518025, which is recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Répondue par: Tech Support Europe
Date de publication: 2021-11-09

what is the working concentration to be used

Posée par: Thara
Thank you for your question. Please refer to our protocols for the recommended concentrations for our antibodies. If you have any further questions, please contact Technical Service. https://www.scbt.com/resources/protocols
Répondue par: Technical Service 3
Date de publication: 2020-11-25

I am getting a band between 37 and 50 kDa with this antibody. Has someone else reported this?

Posée par: Kranti
Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa, but do not have information regarding a smaller size. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.
Répondue par: Technical Support
Date de publication: 2020-11-02

What is the expected molecular weight for bands?

Posée par: JJJJ55
Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.
Répondue par: SCBT Heidelberg Support
Date de publication: 2019-03-07

I performed an immunoprecipitation using this antibody (PGC-1 alpha Antibody - H-300) and I had a problem since the heavy chain (HC, 55 kDa) of the IP antibody were also recognized.

Posée par: johnny86
Thank you for your question. This is rabbit polyclonal antibody, PGC-1α (H-300): sc-13067, is expected to detect the PGC-1α protein at approximately 91 kDa. The heavy chain of IgG is approximately 55 kDa. Therefore, observing the heavy chain IgG band from your immunoprecipitating antibody should not interfere with detection of your target protein. However, if you wish to not observe the heavy chain, then we would recommend using a different antibody for your Western blot. For example, a mouse monoclonal against PGC-1α. Then you could use an anti-mouse IgG secondary antibody, that would not recognize the heavy chain of the rabbit IgG primary used for immunoprecipitation.
Répondue par: Technical Support
Date de publication: 2018-08-17

I used this antibody on cells and get 2 bands very close together which one is pgc1 alpha upper or lower

Posée par: Joey88
Thank you for your question. We are sorry to hear that this antibody did not perform as expected. It is very difficult to say which band is which without having more information. Please reach out to our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody and make sure you get the results you are looking for!
Répondue par: Tech Service
Date de publication: 2017-06-07
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Rated 2 de 5 de par OKI bought this few months ago, it did not perform really well in western blot
Date de publication: 2018-09-28
Rated 5 de 5 de par Highly specific antibodyWe bought this antibody for use in western, IF and IHC and we are highly satisfied by its performance. WB- 1:1000, IF- 1:250, IHC- 1:200
Date de publication: 2017-03-09
Rated 5 de 5 de par Worked well in WBI used this antibody in WB (dilution 1:500), the band is wonderful.
Date de publication: 2016-11-01
Rated 5 de 5 de par worked wellI used this antibody in WB (dilution 1:500), the band is wonderful.
Date de publication: 2016-10-31
Rated 1 de 5 de par Multiple unspecific bands across antibodyMultiple unspecific bands across antibody dilutions (1:100-10:1000) and independent of protein loading (20-80ug). No band at expected size. Mouse liver tissues were used.
Date de publication: 2016-01-12
Rated 4 de 5 de par This has worked well in WBThis has worked well in WB.
Date de publication: 2015-11-04
Rated 5 de 5 de par This has worked well in WBThis has worked well in WB
Date de publication: 2015-10-27
Rated 4 de 5 de par Used in IF and got good staining at 1:100 dilutionUsed in IF and got good staining at 1:100 dilution.
Date de publication: 2015-09-24
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Anticorps PGC-1α (H-300) est évalué 4.0 de 5 de 16.
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