Date published: 2025-9-8

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Anticorps paxillin (D-8): sc-365059

4.5(4)
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Fiches techniques
  • L'Anticorps paxillin (D-8) est un monoclonal de souris IgG1 κ, cité dans 6 publications, fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 17-47 près de N-terminus de paxillin d'origine human
  • recommandé pour la détection de α,β, γ isoforms of paxillin d'origine mouse, rat et human par WB, IP, IF et ELISA; également réactif avec d'autres espèces, dont et equine and canine
  • Voir paxillin (B-2): sc-365379 pour d'autres anticorps conjugués paxillin, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs de détection secondaire préférés pour paxillin Antibody (D-8) for WB applications. Ces réactifs sont désormais proposés en lots avec paxillin Antibody (D-8)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps paxillin (D-8)

sc-365059
200 µg/ml
$316.00

paxillin (D-8): m-IgG Fc BP-HRP Kit

sc-540304
200 µg Ab; 10 µg BP
$354.00

paxillin (D-8): m-IgG1 BP-HRP Kit

sc-542026
200 µg Ab; 20 µg BP
$354.00

paxillin (D-8) peptide neutralisant

sc-365059 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365059 P appropriate for?

Posée par: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 3 de 5 de par Good for WB, but NOT for IF!I bought this to use mainly for IF, because the product sheet IF picture looked good. Western blots do work (so I gave 3 stars), but IF had absolutely no signal. I tested it twice alongside 5 other Santa Cruz mouse monoclonal antibodies and this was the only one that absolutely did not work (4% PFA fixation,1/50 primary antibody dilution). I then tested it in cells with GFP-Zyxin so I could see that adhesions were there but the anti-paxillin staining was not. Super disappointed to have wasted $ on this one.
Date de publication: 2019-04-19
Rated 5 de 5 de par positive western blot staining for paxillinWe used this antibody for paxillin expression detection with western blot. We got a positive band at the correct molecular weight for HeLa cells
Date de publication: 2017-01-31
Rated 5 de 5 de par Excellent immunofluorescence focal adhesionsExcellent immunofluorescence focal adhesions and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date de publication: 2015-01-29
Rated 5 de 5 de par Produced positive Western Blot data of paxillinProduced positive Western Blot data of paxillin expression in CCD-1064Sk whole cell lysate. -SCBT QC
Date de publication: 2014-05-27
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Anticorps paxillin (D-8) est évalué 4.5 de 5 de 4.
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