Date published: 2025-9-9

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Anticorps paxillin (177): sc-136297

4.5(2)
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Fiches techniques
  • L'anticorps paxillin 177 est un monoclonal IgG1 de souris, cité dans 7 publications, fourni en 50 µg/0.5 ml
  • dirigé contre les acides aminés 1-557 correspondant à la séquence totale de paxillin d'origine chicken
  • recommandé pour la détection de paxillin d'origine mouse, rat, human, avian, bovine et canine par WB, IP et IF
  • Voir paxillin (B-2): sc-365379 pour d'autres anticorps conjugués paxillin, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire paxillin (177). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps paxillin (177)

sc-136297
50 µg/0.5 ml
$316.00

I am using paxillin (177): sc-136297 for immunofluorescence, what dilution of this antibody should I use?

Posée par: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 5 de 5 de par Very specific band was seen6X 5 min wash with TBS-T after primary and secondary antibody incubation. Very specific band was seen, no unspecific bands.
Date de publication: 2017-02-02
Rated 4 de 5 de par Robust band seen in WB using human endothelialRobust band seen in WB using human endothelial whole cell lysate. -SCBT QC
Date de publication: 2013-01-11
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Anticorps paxillin (177) est évalué 4.5 de 5 de 2.
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