PARP1 Antibody (5A5): sc-56197

PARP-1 Antibody (5A5) is a mouse monoclonal IgG1 antibody raised against a fragment of human PARP-1 and is reactive with mouse, rat, and human samples, making anti-PARP-1 antibody (5A5) suitable for detection by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). PARP-1, also referred to as poly(ADP-ribose) polymerase-1, ARTD1, Adprt, and Parp1, is a nuclear DNA-binding protein that plays a critical role in DNA repair, chromatin remodeling, and apoptosis. As a nuclear protein, PARP-1 is primarily located within the nucleus, where PARP-1 is crucial for maintaining genomic stability by recognizing DNA damage and facilitating repair processes. PARP-1′s nuclear localization allows direct binding to DNA strand breaks, where PARP-1 catalyzes the transfer of ADP-ribose from NAD+ to various nuclear proteins, including histones and other chromatin-associated proteins, to modulate chromatin structure and signal the need for repair. This activity is particularly important in response to genotoxic stress, as PARP-1 helps prevent mutations and other deleterious effects of unrepaired DNA damage. Furthermore, cleavage of PARP-1 by caspases during apoptosis generates a p89 fragment that translocates to the cytoplasm, marking a key step in programmed cell death. Deficiencies in PARP-1 are linked to increased chromosomal instability, highlighting PARP-1′s essential function in preserving genome integrity. PARP-1 monoclonal antibody (5A5) enables researchers to effectively study the intricate mechanisms of DNA repair, the maintenance of genomic integrity, and apoptosis, making this tool vital for advancements in molecular biology and medical research.

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PARP1 Antibody (5A5) References:

1. Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.  |  Zhang, N., et al. 2020. Cell Death Differ. 27: 2605-2619. PMID: 32139900
2. The Nucleolus and PARP1 in Cancer Biology.  |  Engbrecht, M. and Mangerich, A. 2020. Cancers (Basel). 12: PMID: 32640701
3. The 89-kDa PARP1 cleavage fragment serves as a cytoplasmic PAR carrier to induce AIF-mediated apoptosis.  |  Mashimo, M., et al. 2021. J Biol Chem. 296: 100046. PMID: 33168626
4. The role of PARP1 in neurodegenerative diseases and aging.  |  Mao, K. and Zhang, G. 2022. FEBS J. 289: 2013-2024. PMID: 33460497
5. Deacetylation-dependent regulation of PARP1 by SIRT2 dictates ubiquitination of PARP1 in oxidative stress-induced vascular injury.  |  Zhang, N., et al. 2021. Redox Biol. 47: 102141. PMID: 34555594
6. A Long-Lasting PARP1-Activation Mediates Signal-Induced Gene Expression.  |  Cohen-Armon, M. 2022. Cells. 11: PMID: 35563882
7. PARP1: Liaison of Chromatin Remodeling and Transcription.  |  Zong, W., et al. 2022. Cancers (Basel). 14: PMID: 36077699
8. A PARP1 PROTAC as a novel strategy against PARP inhibitor resistance via promotion of ferroptosis in p53-positive breast cancer.  |  Li, G., et al. 2022. Biochem Pharmacol. 206: 115329. PMID: 36309080
9. PARP1 inhibition protects mice against Japanese encephalitis virus infection.  |  Desingu, PA., et al. 2023. Cell Rep. 42: 113103. PMID: 37676769
10. Nimbolide targets RNF114 to induce the trapping of PARP1 and synthetic lethality in BRCA-mutated cancer.  |  Li, P., et al. 2023. Sci Adv. 9: eadg7752. PMID: 37878693

The preferred PARP1 antibody is PARP1 Antibody (F-2): sc-8007.