PAO CRISPR/Cas9 KO Plasmid (h): sc-404683
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAO CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAO genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAO CRISPR/Cas9 KO Plasmid (h) | sc-404683 | 20 µg | $397.00 |
Overview
Human SMOX encodes polyamine oxidase (PAO), a peroxisomal flavin-dependent enzyme that catalyzes oxidative back-conversion of spermine to spermidine, linking polyamine flux to cellular redox balance. This reaction generates hydrogen peroxide and aldehyde byproducts, connecting SMOX activity to oxidative stress signaling, DNA damage responses, and regulation of proliferation and differentiation. SMOX/PAO intersects with polyamine biosynthesis and catabolism networks that influence chromatin dynamics and translation, and its dysregulation has been associated in the literature with inflammation, neurodegeneration, and cancer-related metabolic remodeling. As a node in polyamine homeostasis, SMOX is frequently examined for its impact on mitochondrial function, ferroptosis susceptibility, and stress-adaptive transcriptional programs.
PAO CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SMOX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SMOX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SMOX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAO protein expression.
This CRISPR knockout system enables efficient generation of SMOX-deficient cell models for investigation of PAO signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SMOX exon(s) critical for PAO function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SMOX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAO CRISPR/Cas9 KO Plasmid (h) and PAO CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SMOX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAO HDR Plasmid (h) and PAO HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SMOX homology arms to support homology-directed repair at defined SMOX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.