Pannexin-2 CRISPR/Cas9 KO Plasmid (h): sc-405925
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pannexin-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pannexin-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pannexin-2 Antibody (2B11): sc-517064
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pannexin-2 CRISPR/Cas9 KO Plasmid (h) | sc-405925 | 20 µg | $397.00 |
Overview
PANX2 encodes pannexin-2, a plasma membrane channel-forming protein that mediates ATP and other small-molecule release to coordinate autocrine and paracrine signaling. Pannexin-2 contributes to purinergic signaling networks that influence intracellular calcium dynamics, inflammasome-associated responses, and neuron–glia communication, linking channel activity to cellular stress and innate immune modulation. Expression is enriched in the nervous system and has been investigated in contexts of neuroinflammation, excitotoxic injury, and altered tumor cell behavior, where extracellular nucleotide signaling can reshape proliferation and microenvironmental cues. These properties make PANX2 a useful target for dissecting how membrane channels integrate metabolic stress with inflammatory and synaptic pathways.
Pannexin-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PANX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PANX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PANX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pannexin-2 protein expression.
This CRISPR knockout system enables efficient generation of PANX2-deficient cell models for investigation of Pannexin-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PANX2 exon(s) critical for Pannexin-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PANX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pannexin-2 CRISPR/Cas9 KO Plasmid (h) and Pannexin-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PANX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pannexin-2 HDR Plasmid (h) and Pannexin-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PANX2 homology arms to support homology-directed repair at defined PANX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.