p57 Kip2 Antibody (3F245): sc-71823

p57 Kip2 Antibody (3F245) is a mouse monoclonal IgG2b antibody that detects p57 Kip2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p57 Kip2, also known as Kip2, is a crucial member of the p21 Waf1/Cip1/p27 family of cyclin-dependent kinase inhibitors, playing a significant role in regulating cell cycle progression by inhibiting several G1 cyclin/Cdk complexes. This inhibition is vital for maintaining proper cell cycle control, particularly in response to cellular stress or DNA damage, as p57 Kip2 prevents uncontrolled cell proliferation. Unlike p21 Waf1/Cip1, p57 Kip2 operates independently of p53 regulation, which allows p57 Kip2 to exert effects even in p53-deficient contexts. The tight-binding nature of p57 Kip2 to cyclin/Cdk complexes underscores p57 Kip2′s potency as a negative regulator of G1 progression, making p57 Kip2 an important target for studies related to cell cycle dysregulation in various diseases, including cancer. With an ability to arrest cells in G1 phase, p57 Kip2 serves as a critical checkpoint in cellular responses to growth signals, thereby influencing both normal development and tumorigenesis.

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p57 Kip2 Antibody (3F245) References:

1. p57(KIP2) is not mutated in hepatoblastoma but shows increased transcriptional activity in a comparative analysis of the three imprinted genes p57(KIP2), IGF2, and H19.  |  Hartmann, W., et al. 2000. Am J Pathol. 157: 1393-403. PMID: 11021841
2. p57(KIP2) immunohistochemical staining of gestational trophoblastic tumours does not identify the type of the causative pregnancy.  |  Sebire, NJ., et al. 2004. Histopathology. 45: 135-41. PMID: 15279631
3. p57(kip2) expression is related to carcinogenesis and tumor progression in laryngeal tissues.  |  Fan, GK., et al. 2006. Acta Otolaryngol. 126: 301-5. PMID: 16618659
4. Expression of p57-Kip2 in monocytes and macrophages.  |  Hofer, TP., et al. 2006. Immunobiology. 211: 455-62. PMID: 16920485
5. Regulation of p57(KIP2) during muscle differentiation: role of Egr1, Sp1 and DNA hypomethylation.  |  Figliola, R., et al. 2008. J Mol Biol. 380: 265-77. PMID: 18513743
6. Significance of p57(Kip2) down-regulation in oncogenesis of bladder carcinoma: an immunohistochemical study.  |  Bozdoğan, O., et al. 2008. Tumori. 94: 556-62. PMID: 18822693
7. p57(Kip2) and p27(Kip1) cooperate to maintain hematopoietic stem cell quiescence through interactions with Hsc70.  |  Zou, P., et al. 2011. Cell Stem Cell. 9: 247-61. PMID: 21885020
8. p57(KIP2) regulates radial glia and intermediate precursor cell cycle dynamics and lower layer neurogenesis in developing cerebral cortex.  |  Mairet-Coello, G., et al. 2012. Development. 139: 475-87. PMID: 22223678
9. Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells.  |  Takahashi, S., et al. 2019. Proc Natl Acad Sci U S A. 116: 26606-26613. PMID: 31792181
10. Determination of morphologic and immunohistochemical stain (p57 kip2) discrepancy of complete and partial hydatidiform mole by using microsatellite genotyping.  |  Oranratanaphan, S., et al. 2020. Taiwan J Obstet Gynecol. 59: 570-574. PMID: 32653131

The preferred p57 Kip2 antibody is p57 Kip2 Antibody (KP39): sc-56341.