p53 Antibody (E-5): sc-74573

p53 Antibody (E-5) is a non-mammalian monoclonal IgG2a kappa light chain antibody, raised against amino acids 186-385 of p53 from Drosophila melanogaster origin, exhibiting species reactivity specifically with Drosophila melanogaster. The p53 protein, known as Dmp53 in Drosophila, is a crucial tumor suppressor and transcription factor that plays a significant role in regulating the cell cycle, DNA repair, and apoptosis in response to cellular stress and DNA damage. Located primarily in the nucleus, Dmp53 binds to DNA and activates the transcription of genes involved in growth arrest and programmed cell death, such as Reaper, Hid, and Grim, thereby influencing cell differentiation and maintaining genomic stability. Nuclear localization of Dmp53 is essential for function; however, Dmp53 can be chaperoned to the cytoplasm by MDM2, an E3 ubiquitin ligase that targets Dmp53 for proteasomal degradation through poly-ubiquitination. This interaction is vital for controlling Dmp53 levels and preventing excessive cell death or uncontrolled cell proliferation. Dmp53′s activity is modulated by posttranslational modifications, including phosphorylation and acetylation, which can alter DNA-binding affinity and transcriptional activity. Mutations or dysregulation in the DNA-binding domain (amino acids 110-286) of Dmp53 can impair tumor suppressor functions, leading to developmental abnormalities and increased sensitivity to genotoxic stress. p53 (E-5) antibody is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), making p53 monoclonal antibody (E-5) a valuable tool for researchers studying function, cellular localization, and regulatory mechanisms of Dmp53 in Drosophila melanogaster.

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.