p53 Antibody (DO-2): sc-53394

p53 Antibody (DO-2) is a mouse monoclonal antibody IgG1 kappa light chain that specifically targets the p53 protein (also designated TP53) of mouse, rat, and human origin. p53 monoclonal antibody (DO-2) recognizes an epitope located between amino acids 10-16 of the p53 protein and is widely used in a variety of applications including western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry on paraffin-embedded tissues. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates, p53 IgG1 kappa monoclonal antibody (DO-2) is ideal for detecting the tumor suppressor p53, a crucial regulator of cell cycle control, apoptosis, and DNA repair. The p53 protein operates by upregulating genes associated with growth arrest and programmed cell death in response to cellular stress signals. It primarily localizes to the nucleus but can be shuttled to the cytoplasm by MDM2, an E3 ubiquitin ligase that regulates p53 by tagging it for proteasomal degradation. This regulation allows p53 to fluctuate between inactive and active states, driven by post-translational modifications such as phosphorylation and acetylation. Mutations in the DNA-binding domain of p53 (amino acids 110-286) can impair its DNA binding capabilities, contributing to various cancers. Anti-p53 antibody (DO-2) is particularly useful in studies of p53′s role in tumor suppression, cell differentiation, and its dynamic regulation under different physiological and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p53 Antibody (DO-2) References:

1. p53 and cell-cycle control: a finger in every pie.  |  North, S. and Hainaut, P. 2000. Pathol Biol (Paris). 48: 255-70. PMID: 10858958
2. P53-responsive genes and the potential for cancer diagnostics and therapeutics development.  |  Xu, H. and el-Gewely, MR. 2001. Biotechnol Annu Rev. 7: 131-64. PMID: 11686042
3. p53 Tumor suppressor protein content in human uterine leiomyomas and its down-regulation by 17 beta-estradiol.  |  Gao, Z., et al. 2002. J Clin Endocrinol Metab. 87: 3915-20. PMID: 12161533
4. Detection of p53 protein transcriptional activity by chromatin immunoprecipitation.  |  Yang-Hartwich, Y., et al. 2015. Methods Mol Biol. 1219: 87-93. PMID: 25308264
5. Flexibility is important for inhibition of the MDM2/p53 protein-protein interaction by cyclic β-hairpins.  |  Danelius, E., et al. 2016. Org Biomol Chem. 14: 10386-10393. PMID: 27731454
6. The structure formed by inverted repeats in p53 response elements determines the transactivation activity of p53 protein.  |  Brázda, V., et al. 2017. Biochem Biophys Res Commun. 483: 516-521. PMID: 28007599
7. Estimating the Effect of Single-Point Mutations on Protein Thermodynamic Stability and Analyzing the Mutation Landscape of the p53 Protein.  |  Banerjee, A. and Mitra, P. 2020. J Chem Inf Model. 60: 3315-3323. PMID: 32401507
8. Computational molecular characterization of p53 and Human TLRs interactions.  |  JahoorAlam, M., et al. 2022. Cell Mol Biol (Noisy-le-grand). 67: 1-5. PMID: 35818278
9. Multifunctional metallochaperone modifications for targeting subsite cavities in mutant p53-Y220C.  |  Miller, JJ., et al. 2023. J Inorg Biochem. 242: 112164. PMID: 36871418
10. Design of a synthetic Mdm2-binding mini protein that activates the p53 response in vivo.  |  Böttger, A., et al. 1997. Curr Biol. 7: 860-9. PMID: 9382809

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (DO-2) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (DO-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (DO-2) Specifications

Alternate Product Name: TP53

Product Clone Name: DO-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 10-16

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: p53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (DO-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.